Objective To find out the status and influencing factors of unintentional injury in preschool children of Chengguan district in Lhasa city, and to provide basis for health education of childern′s unintentional injury.Methods A total of 611 preschool children from one public and two private kindergartens in Chengguan district of Lhasa were investigated with a self-designed questionnaire on children′s unintentional injury. Results The unintentional injury rate of preschool children was 36.33%. The top three types of unintentional injury were falls, sprains, and dislocations(45.97%), animal bites(18.55%) and burns(10.89%). The top three of occurrence places were home(36.70%), activity sites outside schools(19.59%) and road(14.10%). The Chi-square test showed that the prevalence of unintentional injury among preschoolers of different nationality, genders, mothers′ literacy level and living floors were statistically significant(P<0.05). Multivariate non-conditional Logistic regression analysis showed that children′s nationality, gender and residential floor were the factors affecting children′s unintentional injuries. Conclusions The prevalence rate of unintentional injury in preschool children of Chengguan district in Lhasa City is high. The unintentional injury of Han nationality is higher than that of other Tibetan ethnic groups. Also, the intentional injury in children who are boys, and dwelled in the place higher than the third floor is more prevalent. Therefore, relevant departments should pay attention to the intentional injury status of preschool children in Lhasa, and actively take preventive measures.