Effect of rhEPO treatment on MOG expression in brain tissue of newborn rats with intrauterine inflammation of remyelination and its correlation with myelin repair

SUN Meng-ya; LUO Gang; WANG Qian; JIANG Hong

doi:10.11852/zgetbjzz2018-26-04-09

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Chinese Journal of Child Health Care ›› 2018, Vol. 26 ›› Issue (4) : 381-385. DOI: 10.11852/zgetbjzz2018-26-04-09

Effect of rhEPO treatment on MOG expression in brain tissue of newborn rats with intrauterine inflammation of remyelination and its correlation with myelin repair

SUN Meng-ya¹, LUO Gang², WANG Qian¹, JIANG Hong¹

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Abstract

Objective To study the neuroprotective effect of rhEPO on brain tissue of newborn rats with intrauterine infection by detecting the changes of MOG expression,in order to provide reference for clinical intervention. Methods 1)Wistar rats that had been pregnant for 15 days were selected and divided into LPS infection group and control group through intraperitoneal injection of LPS (0.3 mg/kg) and equivalent stroke-physiological saline solution.Totally 64 newborn rats in the same fetus of the LPS infection group with brain inflammatory injury proved by HE staining were randomly selected and were divided into rhEPO treatment group (n=32) and infection control group(n=32).Meantime,32 newborn rats in the control group were randomly enrolled as the blank control group.Three groups of rats were conducted intraperitoneal injection of rhEPO (5000IU/kg) or equivalent saline solution simultaneously promptly every day after the birth.2) Brain tissues of three groups were took out by perfusing formaldehyde on 0h,the 3rd,7th and 14th of their birth respectively.And the levels of MOG mRNA and MOG protein were tested by RT-PCR and western blot respectively. Results 1)The levels of MOG mRNA of blank control group were significantly higher on the 3rd (2.05±0.75),7th (2.37±0.57) and 14th (3.36±0.81) day than those on 0 hour (1.01±0.16)(P＜0.05).2)The levels of MOG mRNA on 0 hour,the 3rd,7th and 14th day of infection control group were lower than those of blank control group,and the differences were significant on the 3rd and 7th day.3)The levels of MOG mRNA on the 3rd(2.18±0.89),7th(3.53±1.55) and 14th(3.30±1.12) were significantly higher in rhEPO treatment group than those in infection control group(P＜0.05).4)The level of MOG protein in rhEPO treatment group was significantly higher than that in the infection control group with the same age on the 3rd(18.23±0.37 vs12.63±0.65),7th(22.72±2.77 vs 13.59±1.10),and 14th day(25.85±2.70 vs15.63±1.32). Conclusion The mechanism of inhibiting the expression of MOG gene and protein in the myelin sheath was found in the brain tissue damage induced by intrauterine inflammation in neonatal rats.Early application of rhEPO can promote the expression of MOG gene and protein,to play a protective role in nerve repairment.The best treatment may last for 14 days.

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intrauterine inflammation / newborn / brain injury / erythropoietin / myelin oligodendrocyte glycoprotein

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SUN Meng-ya, LUO Gang, WANG Qian, JIANG Hong. Effect of rhEPO treatment on MOG expression in brain tissue of newborn rats with intrauterine inflammation of remyelination and its correlation with myelin repair[J]. Chinese Journal of Child Health Care. 2018, 26(4): 381-385 https://doi.org/10.11852/zgetbjzz2018-26-04-09

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