Effect of ω-3 PUFAs on regulation of Toll-like receptor 4 and nuclear factor-ΚB signaling pathway in neonatal rats with brain injury induced by LPS

SHI Ji-peng; LI Yan-wei; SONG Ya-hui; ZHANG Li-li; SANG Gui-mei; GUO Hong-xun; WANG Wei-wei; TANG Cheng-he; Huang Li-mi

doi:10.11852/zgetbjzz2018-26-02-12

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Chinese Journal of Child Health Care ›› 2018, Vol. 26 ›› Issue (2) : 158-162. DOI: 10.11852/zgetbjzz2018-26-02-12

Effect of ω-3 PUFAs on regulation of Toll-like receptor 4 and nuclear factor-ΚB signaling pathway in neonatal rats with brain injury induced by LPS

SHI Ji-peng¹, LI Yan-wei², SONG Ya-hui³, ZHANG Li-li, SANG Gui-mei¹, GUO Hong-xun¹, WANG Wei-wei¹, TANG Cheng-he¹, Huang Li-mi⁴

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Abstract

Objective To investigate the effects of ω-3 PUFAs on regulation of Toll-like receptor 4 (TLR4) and nuclear factor-κB (NF-κB) signaling pathway in neonatal rats with brain injury induced by LPS. Methods Totally 96 neonatal Sprague-Dawley rats were divided into control group,ω-3 group,ω-6 group,and LPS group.Intraperitoneal injection with saline or LPS was administered and immediately saline or fat emulsions was injected to establish a model of rat with brain injury induced by infection and the duration time was 1 day or 5 days respectively.Those groups were sacrificed at 1d or 5 d after intraperitoneal injection with saline or fat emulsions.Expression levels of TLR4 mRNA and NF-κB mRNA were examined by real-time PCR,meanwhile the levels of TLR4,and NF-κB in the hippocampus were measured by Western Blot. Results The expressions of TLR mRNA and NF-κB mRNA and the protein level of TLR4 and NF-κB in the hippocampus were significantly different in four groups(all Pˉ0.05).TLR mRNA and NF-κB mRNA and the protein expression levels in ω-3 group were lower than those in ω-6 group and LPS group on 1 d after injection (all Pˉ0.05).And the protein expression levels of TLR mRNA and NF-κB mRNA in ω-3 group were lower than those in ω-6 group and LPS group on five days after injection (all Pˉ0.05).No matter the first day or the fifth day,the expression of TLR mRNA and NF-κB mRNA and the level of TLR4 and NF-κB in ω-3 group were significantly lower than those in ω-6 group(all Pˉ0.05). Conclusion ω-3 PUFAs can down regulate the activity of signaling pathway of TLR4 and NF-κB,so it may has an anti-inflammatory effect on brain injuryinduced by LPS.

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neonatal rats / ω-3 PUFAs / ω-6 PUFAs / brain injury / LPS / TLR4 / NF-κB

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SHI Ji-peng, LI Yan-wei, SONG Ya-hui, ZHANG Li-li, SANG Gui-mei,
GUO Hong-xun, WANG Wei-wei, TANG Cheng-he, Huang Li-mi. Effect of ω-3 PUFAs on regulation of Toll-like receptor 4 and nuclear factor-ΚB signaling pathway in neonatal rats with brain injury induced by LPS[J]. Chinese Journal of Child Health Care. 2018, 26(2): 158-162 https://doi.org/10.11852/zgetbjzz2018-26-02-12

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