Study on the correlation between serum microRNA 20a and inflammatory response in children with pneumonia

LIU Chun-yan; KE Wen-ling; CHAI Lin; ZHANG Hui

doi:10.11852/zgetbjzz2018-1434

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Chinese Journal of Child Health Care ›› 2019, Vol. 27 ›› Issue (8) : 881-884. DOI: 10.11852/zgetbjzz2018-1434

Study on the correlation between serum microRNA 20a and inflammatory response in children with pneumonia

LIU Chun-yan¹, KE Wen-ling¹, CHAI Lin², ZHANG Hui¹

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Abstract

Objective To explore the correlation between serum microRNA 20a and the progress of inflammatory response in children with pneumonia,in order to provide reference for clinical treatment.Methods Totally 90 children diagnosed with pneumonia from March 2015 to February 2017 were selected as pneumonia group (n=90) and 90 healthy children were enrolled in control group (n=90).Routine blood test and erythrocyte sedimentation rate were conducted in children after admission,and specific antibodies against pneumonia were identified by ELISA.Serum concentration of C-reactive protein (CRP) and rheumatoid factor (RF) was quantitatively detected by automatic specific protein analyzer and immunoturbidimetry.Total RNA was separated by TRIzol reagent and reverse transcription cDNA was synthesized from microRNA 20a in serum according to Prime-Script RT kit instructions.Then,the protein extracts were separated by 10%SDS-PAGE gel electrophoresis and transferred to PVDF membrane to express microRNA 20a protein in 20a.Finally,Kolmogorov-Smirnov test was used to analyze the correlation between the expression of microRNA 20a in serum and inflammatory response in children with pneumonia. Results Compared with the control group,serum levels of IgG and IgA in children with pneumonia increased,but IgM did not change significantly.Specific protein analysis showed that serum CRP,RF and IL-17 levels and the expression level of microRNA 20a in pneumonia group were higher than those in control group (P<0.05).The correlation analysis between microRNA 20a parameters and IL-17 level showed positive correlation and a steep correlation curve (r=0.571,P＝0.002). Conclusions Serum expression level of microRNA 20a may be related to the pathogenesis of pneumonia inflammation.MicroRNA 20a is promising to be a marker for the diagnosis of pediatric pneumonia and can be used as an indicator for the poor prognosis of pediatric pneumonia patients.

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microRNA 20a / pediatric pneumonia / inflammatory reactions

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LIU Chun-yan, KE Wen-ling, CHAI Lin, ZHANG Hui. Study on the correlation between serum microRNA 20a and inflammatory response in children with pneumonia[J]. Chinese Journal of Child Health Care. 2019, 27(8): 881-884 https://doi.org/10.11852/zgetbjzz2018-1434

References

[1] 唐曦,胡娅,徐炎华,等.MicroRNA-21、microRNA-137和microRNA-498在肺腺癌患者血清中表达情况及其临床意义[J].中国现代医学杂志,2018,28(4):39-44.
[2] 王辉,朱海燕.血清营养素水平与肺炎患儿免疫细胞分化、炎症反应激活的相关性[J].海南医学院学报,2018,24(10):1015-1018.
[3] 沈开慧,张孟孝,周金玲,等.病毒性脑炎小儿血清VCAM-1含量检测及神经损伤、炎症反应的相关关系[J].海南医学院学报,2018,24(4):560-562,566.
[4] 周轶冰.肝细胞癌患者血清中microRNA-21和microRNA-126的表达及临床意义[J].中国现代医药杂志,2018,20(4):47-49.
[5] 柴巧利.手足口病并发脑炎患儿血清NSE、S100β含量与炎症反应、免疫应答的相关性[J].海南医学院学报,2017,23(163):1815-1818.
[6] Hasan DM,Rasoul B,Abbas AV,et al.Elevated IL-17 and TGF-β serum levels: A positive correlation between T-helper 17 cell-related pro-inflammatory responses with major depressive disorder[J].Basic & Clinical Neuroscience,2016,7(2):137-142.
[7] 张岩,王聪霞,李永勤,等.血浆MicroRNA-17-5p和microRNA-20水平与肺动脉高压预后的相关性研究[J].河北医学,2017,23(8):1236-1241.
[8] 马箐.MicroRNA-320c、MicroRNA-451a、MicroRNA-486在食管鳞癌及癌前病变患者血清中的表达及其作为初筛标志物的可行性研究[D].北京:北京协和医学院,2017.
[9] 刘运龄.microRNA-155对动脉粥样硬化免疫炎症反应的影响[D].天津:天津医科大学,2017.
[10] 熊欣.microRNA-214在人胃腺癌组织和血浆中的表达、临床意义及其对抗肿瘤免疫抑制作用的研究[D].南昌:南昌大学,2017.
[11] 邵慧芝,吕勤,陈赫赫,等.microRNA-223在重症肺炎患儿血浆中的表达意义及其与预后的关系[J].中国医师杂志,2016,18(3):402-409.
[12] 姜威.凋亡通路基因的microRNA相关单核苷酸多态性与局限期小细胞肺癌预后的关联研究和调强放疗与三维适形放疗技术对局部晚期非小细胞肺癌放射性肺损伤影响的临床研究[D].北京:北京协和医学院中国医学科学院;北京协和医学院;中国医学科学院;清华大学医学部,2016.
[13] 彭晶,袁瑞丽,王晓琴,等.多发性骨髓瘤患者外周血清中microRNA-181a和microRNA-20a表达的检测及其临床意义[J].吉林大学学报:医学版,2015,41(3):625-630.
[14] Liang Y,Chang C,Zhu H,et al.Correlation between decrease of CRP and resolution of airway inflammatory response,improvement of health status,and clinical outcomes during severe acute exacerbation of chronic obstructive pulmonary disease[J].Intern Emerg Med,2015,10(6):1-7.
[15] 陈珊珊,井宣,涂建成.血清microRNA作为乙型肝炎病毒相关肝细胞癌发生过程标志物的研究进展[J].武汉大学学报:医学版,2015,36(4):653-658.
[16] 钟广芝,胡鹏飞.血液透析患者血清铁调素、转铁蛋白受体含量与铁缺乏、微炎症反应的相关性[J].海南医学院学报,2018,24(1):30-33.
[17] 贺钰梅,韩秀平,汪丽丽,等.痛风性关节炎患者血清Dickkofpf-1含量与骨破坏、炎症及氧化反应的相关性研究[J].海南医学院学报,2017,23(17):2357-2360.
[18] 沈金闻,刘鲁迎,李德川,等.术前系统性炎症反应与结肠癌临床病理特征的相关性[J].中国现代医学杂志,2016,26(21):87-93.
[19] 陈曦,焦蓉,常立文.重症肺炎患儿肠道群紊乱与全身炎症反应及应激反应程度的相关性[J].海南医学院学报,2018,24(13):1272-1275.
[20] Derrick T,Last AR,Burr SE,et al.Inverse relationship between microRNA-155 and -184 expression with increasingconjunctival inflammation during ocular Chlamydia trachomatis infection.[J].Bmc Infectious Diseases,2015,16(1):60-71.