Association between interleukin-8 gene polymorphism and occurrence and development of acute bronchitis induced by respiratory syncytial virus

DU Lin-bo; WANG Hong-bing; LIAO Fan-da; LIU Dan-hong; WANG Xuan

doi:10.11852/zgetbjzz2018-1045

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Chinese Journal of Child Health Care ›› 2019, Vol. 27 ›› Issue (11) : 1232-1235. DOI: 10.11852/zgetbjzz2018-1045

Association between interleukin-8 gene polymorphism and occurrence and development of acute bronchitis induced by respiratory syncytial virus

DU Lin-bo, WANG Hong-bing, LIAO Fan-da, LIU Dan-hong, WANG Xuan

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Abstract

Objective To investigate the association between interleukin-8 gene polymorphism and occurrence and development of acute bronchitis induced by respiratory syncytial virus,in order to provide scientific reference for predicting the development of acute bronchitis. Methods Totally 80 children with acute bronchitis induced by respiratory syncytial virus(case group) and 30 healthy children (control group) who underwent physical examination were selected as the participants from March to September in 2017.According to the severity of the disease,children in case group were divided into mild group (n=52) and moderate to severe group (n=28).The expression of IL-8 in three groups was tested by enzyme linked immunosorbent assay (ELISA),and the genotype frequencies of-251 and 781 polymorphic loci of three groups of IL-8 genes were measured by PCR-RFLP. Results The serum expression of IL-8 in control group [(4.35±0.84)pg/ml] was lower than that in the other two groups.The expression of IL-8 in mild group was lower than that in moderate and severe group [(7.63±1.31)pg/ml vs.(10.22±1.85)pg/ml,P<0.05)].The AA genotype frequency of IL-8 gene-251 locus in control group (6.67%) was lower than that in the other two cases (23.08%,28.57%) (P<0.05).Likewise,the frequency of allele A of IL-8 gene-251 locus in control group (33.33%) was lower than that in the other two groups (51.92%,55.38%) (P<0.05).Linkage analysis indicated that the A allele of the IL-8 gene-251 loci was closely linked to the 781 locus C allele,and the common AC haplotype was associated with the susceptibility to acute bronchitis caused by respiratory syncytial virus and the severity of the disease. Conclusion The AC haplotype of-251 and 781 loci of IL-8 gene is associated with the occurrence and development of acute bronchitis induced by respiratory syncytial virus.

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interleukin-8 / singlenucleotidepolymorphisms / respiratorysyncytialvirus / acutebronchitis

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DU Lin-bo, WANG Hong-bing, LIAO Fan-da, LIU Dan-hong, WANG Xuan. Association between interleukin-8 gene polymorphism and occurrence and development of acute bronchitis induced by respiratory syncytial virus[J]. Chinese Journal of Child Health Care. 2019, 27(11): 1232-1235 https://doi.org/10.11852/zgetbjzz2018-1045

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