Study of the intestinal microbial diversity in obese children by illumina amplicon sequencing of 16SrDNA Tag

WU Ya; FANG Yuan-yuan; CHEN Yan-hui; YANG Li-qi; WANG Jian-qing

doi:10.11852/zgetbjzz2018-1011

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Chinese Journal of Child Health Care ›› 2018, Vol. 26 ›› Issue (12) : 1297-1300. DOI: 10.11852/zgetbjzz2018-1011

Study of the intestinal microbial diversity in obese children by illumina amplicon sequencing of 16SrDNA Tag

WU Ya¹, FANG Yuan-yuan¹, CHEN Yan-hui¹, YANG Li-qi², WANG Jian-qing²

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Abstract

Objective To study the composition and characteristics of intestinal microorganisms in obese children by illumina amplicon sequencing of 16SrDNA Tag, in order to provide basis for further related research. Methods Totally 59 obese children and children with normal weight were enrolled in this study, and their feces were collected.16SrDNA method was used to detect the composition and difference of intestinal flora in different groups. Results There was no significant difference on intestinal microbial diversity between obese and normal weight children(P>0.05), but significant differences on distribution and quantity were found(P<0.05).At the phylum level, Firmicutes, Bacteroidetes and Acidobacteria were the most abundant flora in children′s intestinal microbial community.The number of Verrucomicrobia in normal weight group was significantly higher than that in obese group.At the genus level, it was found that the number of Dorea was significantly higher in obese group than that in normal weight group, while the number of Akkmermansia was significantly higher in normal weight children. Conclusion The change of intestinal flora distribution pattern and quantity is closely related to the occurrence of obesity.

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childhood obesity / intestinal microbial / 16SrDNA amplicon sequencing

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WU Ya, FANG Yuan-yuan, CHEN Yan-hui, YANG Li-qi, WANG Jian-qing. Study of the intestinal microbial diversity in obese children by illumina amplicon sequencing of 16SrDNA Tag[J]. Chinese Journal of Child Health Care. 2018, 26(12): 1297-1300 https://doi.org/10.11852/zgetbjzz2018-1011

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