Objective To investigate the characteristics of auditory brainstem response(ABR) in normal infants aged 1~6 months,and to establish a normal range of the peak latencies(PLs) of wave Ⅰ、Ⅲ、Ⅴ and interpeak latencies(IPLs) of Ⅰ-Ⅲ、Ⅲ-Ⅴ、Ⅰ-Ⅴ, in order to provide basis for clinic work. Methods Totally 328 infants with normal hearing and 40 adults were tested by ABR from January 2017 to January 2018. The PLs of wave Ⅰ、Ⅲ、Ⅴ and IPLs of Ⅰ-Ⅲ、Ⅲ-Ⅴ、Ⅰ-Ⅴ from infants of different age groups and ears on different sides were compared. Results The PL of wave I in infants aged 1-6 months was(1.65±0.07),(1.59±0.07),(1.59±0.05),(1.57±0.07),(1.56±0.05) ms and(1.54±0.06) ms,respectively. And the value at the age of 5 months was close to that of adults [(1.53±0.02)ms,P>0.05)]. The PLs of wave Ⅲ、Ⅴ and the IPLs of Ⅰ-Ⅲ、Ⅲ-Ⅴ、Ⅰ-Ⅴ became shorter with the increase of age,which were(4.04±0.12),(6.11±0.13),(2.50±0.11),(2.07±0.13)ms and(4.57±0.12)ms respectively in infants of 6 months old. But these values were still significantly longer than those of adults[(3.90±0.08),(5.77±0.07),(2.37±0.08) ms,(1.87±0.11)ms and(4.24±0.07)ms,P<0.05)]. No significant differences were found between ages or different side of ears(P>0.05). Conclusion The establishment of normalrange of ABR wave for the infants aged 1 to 6 months will provide reliable basis for early diagnosis and follow-up detection of hearing and development disorders.