Objective To explore the impact of the assisted reproductive technology(ART) on the offspring infants' assessment results of Peabody Developmental Motor Scale Ⅱ(PDMS-2) test by applying propensity score matching,in order to provide references for judging the effects of ART on offspring's motor development. Methods Infants who had received PDMS-2 test without pneumonia,diarrhea,fever and other diseases within the last two weeks,and with no congenital diseases that affect motor development were selected in Minhang District of Shanghai from December 2014 to April 2018.The objects were divided into natural conceived group and ART conceived group,with 216 and 158 participants,respectively.The two groups were matched by applying one-to-one propensity score matching(PSM),setting the matching tolerance to 0.002 7.And the matching covariates included birth weight,gestational age,fetus number,gender,the history of rescue,jaundice,brain and other disease,parental age,education degree,occupation,maternal fetal history of miscarriage,medication,diabetes,hypertension and other diseases,and the assessment age of PDMS-2.The differences between natural conceived group and ART conceived group were compared before and after match. Results There was no statistical difference on all the matching variables,gross motor quotient,original score of each subtest of gross motor and grasp subtest between natural conceived group and ART conceived group after match(P>0.05).And the score of fine motor quotient and original score of visual motor integration subtest in ART group were significantly lower than those of natural conceived group(P<0.05). Conclusion ART may have lower fine motor quotient because of the negative influence of visual motor integration in infants,and further research is warranted.