Objective To investigate knowledge, attitudes and current behaviors regarding nutrition and food safety among junior middle school students, in order to provide suggestions for the future development of nutrition and food safety education. Method By using cluster random sampling and qualitative research, a total of 2 652 students were interviewed using questionnaires from 29 schools in Beijing, Chongqing, Haerbin and Hubei Province from November 2016 to October 2017. Results The average score on knowledge was 9.54±1.866, with the awareness rate was 81.1%; 97.2% of the students agreed that it was important to provide nutrition and food safety education in school;73.6% of the students ate breakfast everyday,12.9% of students drunk sugared beverages everyday in the past week,31.1% of the students never or seldom see the food packaging.Multivariate analysis showed that student receiving health education had higher score of knowledge, attitude and behaviors regarding nutrition and food safety. Conclusion The knowledge and behaviors regarding nutrition and food safety of junior middle school students need to be improved, and school health education will be an effective way to improve students′ health knowledge and behaviors.