Objective To analyze the effect of zinc supplementation on the levels of zinc, immune globulin, complements and inflammatory cytokines of children with rotavirus enteritis(RE). Methods A total of 82 children with RE were randomly divided into the zinc supplementation group (42 cases) and RE group (40 cases) from October 2015 to October 2017.The routine treatment group was given conventional therapy and the zinc supplementation treatment group was treated with oral zinc gluconate tablets 20 mg/d for 14 days additionally.And 102 healthy children were collected as control group at the same time.The levels of zinc, immune globulin, complements and inflammatory cytokines among three groups were tested and compared after treatment. Results Compared with control group, the levels of zinc, immunoglobulin G(IgG), immunoglobulin A(IgA), immunoglobulin M(IgM), complement 3(C3), complement 4(C4) and interleukin-2(IL-2) were obviously lower in children with RE before treatment (P<0.05), while the levels of tumor necrosis factor alpha(TNF-α) and interleukin-8 (IL-8) increased in two RE groups(P<0.05).After treatment, the levels of zinc, IgG, IgA, IgM, C3, C4 and IL-2 had significantly increased (P<0.05), while the TNF-α and IL-8 decreased in two RE groups (P<0.05).Compared with the routine treatment group, the levels of zinc, IgG, IgA, IgM, C3, C4 and IL-2 increased after treatment(P<0.05),while the TNF-α and IL-8 decreased in the zinc supplementation group(P<0.05).Moreover, the duration of symptom in the zinc supplementation group was significantly lower than that in the routine treatment group (P<0.05).There were no obvious adverse reactions in two RE groups during treatment. Conclusion Zinc deficiency is prevalent in children with RE, with decreasing level of IgG, IgA, IgM, C3, C4 and increasing level of inflammatory factors.Zinc supplementation can increase serum levels of zinc, IgG, IgA, IgM, C3, C4 and IL-2, decrease serum TNF-α, IL-8 and the duration of symptoms.