Study on the levels of interleukin 1 beta and interleukin-6 in brain tissue of mice infected with enterovirus type 71

LV Tie-gang; WANG Xiao-mei; LI Qiu-bo; CHEN Zong-bo

doi:10.11852/zgetbjzz2017-25-12-10

PDF(539 KB)

Chinese Journal of Child Health Care ›› 2017, Vol. 25 ›› Issue (12) : 1222-1224. DOI: 10.11852/zgetbjzz2017-25-12-10

Study on the levels of interleukin 1 beta and interleukin-6 in brain tissue of mice infected with enterovirus type 71

LV Tie-gang¹, WANG Xiao-mei¹, LI Qiu-bo^{2, 3}, CHEN Zong-bo ²

Author information +

History +

Abstract

Objective To detect the changes of interleukin 1 beta (IL-1β) and interleukin 6(IL-6) level in the brain tissue of mice infected with enterovirus type (EV71),and to explore the possible pathogenesis of central lesion caused by EV71 infection. Methods Totally 120 one-day-old ICR mice were divided into infection group and control group.EV71 infected mice model was established by intraperitoneal injection.Fluorescence quantitative RT-PCR technique was used to detect the viral load of EV71 in the brain.Pathological changes on brain tissues were observed through hematoxylin-eosin staining.Enzyme linked immunosorbent assay (ELISA) was used to detect the content of IL-6 and IL-1β in brain homogenate. Results The level of IL-1β was significantly higher in infection group at 3,5 and 7 days compared with that in control group (P<0.01).The level of IL-6 was significantly higher in infection group at 3,5,7 and 10 day compared with that in control group(P<0.01).However,no significant difference was observed in the level of IL-1β and IL-6 in infection group at 1,7,10 and 14 days compared with those in control group (P>0.05). Conclusion The content of IL-1 and IL-6 in brain tissue of mice infected with EV71 was significantly increased,and the immune response mediated by IL-1β and IL-6 may be one of the possible mechanisms of CNS damage.

Key words

enterovirus 71 / mice / brain tissue / interleukin 1 beta / interleukin 6

Cite this article

EndNote

Ris (Procite)

Bibtex

Download Citations

LV Tie-gang, WANG Xiao-mei, LI Qiu-bo, CHEN Zong-bo. Study on the levels of interleukin 1 beta and interleukin-6 in brain tissue of mice infected with enterovirus type 71[J]. Chinese Journal of Child Health Care. 2017, 25(12): 1222-1224 https://doi.org/10.11852/zgetbjzz2017-25-12-10

References

[1] Xing J,Liu D,Shen S,et al.Pathologic Studies of Fatal Encephalomyelitis in Children Caused by Enterovirus 71[J].Am J Clin Pathol,2016,146(1):195-106.
[2] 韦丹,蒋敏,欧维琳,等.感染肠道病毒71型14例死亡病例病理特征与临床分期反思[J].中国循证儿科杂志,2013,8(2):81-86.
[3] Wang C,Zhou R,Zhang Z,et al.Intrinsic apoptosis and proinflammatory cytokines regulated in human astrocytes infected with enterovirus 71[J].J Gen Virol,2015,96(10):3010-3022.
[4] Wang SM,Lei HY,Liu CC.Cytokine immunopathogenesis of enterovirus 71 brain stem encephalitis[J].Clin Dev Immunol,2012,876241. [5] Weng KF,Chen LL,Huang PN,et al.Neural pathogenesis of enterovirus 71 infection[J].Microbes Infect,2010,12(7):505-510.
[6] Han J,Wang Y,Gan X,et a1.Serum cytokine profiles of children with human enterovirus 71-associated hand,foot,and mouth disease[J].J Med Virol,2014,86(8):1377-1385.
[7] Ye N,Gong X,Pang LL,et al.Cytokine responses and correlations thereof with clinical profiles in children with enterovirus 71 infections[J].BMC Infect Dis,2015,15:225.
[8] 黄小霏,檀卫平,江润昌.手足口病患儿血清IL-6、IL-10、IL-17水平的变化及其临床意义[J].中山大学学报:医学科学版,2012,33(2):195-197.
[9] SM Wang,HY Lei,CC Liu.Cytokine immunopathogenesis of enterovirus 71 brain stem encephalitis[J].Clin Dev Immunol,2012:876241.
[10] Zhang Y,Liu H,Wang L,et al.Comparative study of the cytokine/chemokine response in children with differing disease severity in enterovirus 71-induced hand,foot,and mouth disease[J].PLoS One,2013,8(6):e67430.
[11] Wallace KL,Marshall MA,Ramos SI,et a1.NKT cells mediate pulmonary inflammation and dysfunction in murine sickle cell disease through production of IFN-gamma and CXCR3 chemokines[J].Blood,2009,114(3):667-676.
[12] Chen Z,Li R,Xie Z,et al.IL-6,IL-10 and IL-13 are associated with pathogenesis in children with Enterovirus 71 infection[J].Int J Clin Exp Med,2014,7(9):2718-2723.
[13] Cheng HY,Huang YC,Yen TY,et a1.The correlation between the presence of viremia and clinical severity in patients with enterovirus 71 infection:a multi-center cohort study[J].BMC Infect Dis,2014,14:417.
[14] Khong WX,Foo DG,Trasti SL,et al.Sustained high levels of interleukin-6 contribute to the pathogenesis of enterovirus 71 in a neonate mouse model[J].J Virol,2011,85:3067-3076.
[15] 卫生部手足口病临床专家组.肠道病毒7l型(EV71)感染重症病例临床救治专家共识(2011年版)[J].中华儿科杂志,2011,49(9):675-678.
[16] Zhang G,Wang J,Yao G,et al.Efficacy of high-dose methylprednisolone pulse therapy in the treatment of enterovirus 71 encephalitis[J].Pak J Pharm Sci,2016,29(4 Suppl):1421-1427.
[17] Wang SM,Lei HY,Huang MC.Enterovirus 71 infection-associated acute flaccid paralysis:a case series of long-term neurologic follow-up[J].J Child Neurol,2014,29(10):1283-1290.
[18] Lee KY.Enterovirus 71 infection and neurological complications[J].Korean J Pediatr,2016,59(10):395-401.