Objective To compare the results of cow's milk challenge test and serum specific antibody detection,and to evaluate the value of cow's milk challenge test on cow's milk allergy. Methods A total of 57 infants with suspected cow's milk allergy received cow's milk challenges in Children's Hospital of Soochow University from February,2015 to November,2015.Blood routine,serum total IgE antibody,serum milk protein specific IgE and serum milk protein specific IgG were tested using enzyme-linked immune method (ELISA) and western blotting.The blood changes of cow's milk challenges positive group were and negative group were compared.The relationship among serum total IgE antibody,serum milk protein specific IgE,serum milk protein specific IgG and the results of the cow's milk challenge were analyzed. Results A total of 57 cases of children with challenges induced positive results in 23 cases (40.4%),while negative results in 34 cases(59.6%).The changes in the count of white blood cell,neutrophil ratio,eosinophils ratio and platelet were studied,however.there were no statistical difierences between the challenge failed group and the passed ones.It was interesting that the neutrophil ratio was higher than before among children who passed the challenge.There was no significant difference in total IgE,serum cow's milk protein IgE and IgG between challenge positive group and negative group. Conclusions The detection of total serum IgE and serum milk protein IgE in infants with suspected cow's milk allergy is of little significance,so testing for IgG against cow's milk is not recommended as a diagnostic tool.It is important and necessary to perform cow's milk protein challenge in diagnosis of infant cow's milk allergy.
Key words
cow&apos /
s milk protein allergy /
serum specific antibody /
cow&apos /
s milk challenge /
infant
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