Objective To investigate the effect of Tea Polyphenols(TP) on lipopolysaccharide (LPS)-induced human bronchial epithelial cell injury and underlying mechanism. Methods Cultured human bronchial epithelial cells (16HBE) were treated with LPS (1,25,50,100 μg/ml) and TP (100,200,400,800 g/ml),respectively.Cell proliferation was measured by MTT method.Cell apoptosis was detected by flow cytometry assay.The expression levels of nuclear NFκB and NFκB were measured by western blot.The content of IL-6,IL-13 and TNF-α were detected by Elisa method.The MDA content,LDH content,SOD activity were measured by corresponding kit. Results Compared with the control group,the cell proliferation was significantly decreased; cell apoptosis was increased;the content of IL-6,IL-13,TNF-α were increased;protein expression of nuclear NFκB,NFκB were increased; the content of MDA,LDH were increased,activity of SOD was decreased.LPS induced 16HBE injury was improved by TP treatment. Conclusion tea polyphenols attenuates lipopolysaccharide-induced human bronchial epithelial cell injury through inhibiting oxidative stress.
Key words
tea polyphenols /
human bronchial epithelial cell /
lipopolysaccharide /
oxidative stress
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
References
[1] Gumeniuk S,Barkhina T,Polner S,et al.Morphofunctional study of bronchial asthma with extrahospital pneumonia[J].Eur Respir J,2014,44(Suppl 58):1034.
[2] Juncadella IJ,Kadl A,Sharma AK,et al.Apoptotic cell clearance by bronchial epithelial cells critically influences airway inflammation[J].Nature,2013,493(7433):547-551.
[3] 刘鑫,刘穆华,罗晓青,等.柴朴汤含药血清对支气管上皮细胞TLR-4及NF-κB表达的影响[J].现代医学,2015,43(8):951-956.
[4] Cheng Q,Wang Z,Ma R,et al.Lipoxin A4 protects against lipopolysaccharide-induced sepsis by promoting innate response activator B cells generation[J].Int Immunopharmacol,2016,39(2):29-35.
[5] Xu C,Yang B,Zhu W,et al.Characterisation of polyphenol constituents of Linderae aggregate leaves using HPLC fingerprint analysis and their antioxidant activities[J].Food Chem,2015,186(8):3-9.
[6] Leinaar E,Alamian A,Wang L.A systematic review of the relationship between asthma,overweight,and the effects of physical activity in youth[J].Ann Epidemiol,2016,26(7):227-238.
[7] Ling KM,Sutanto EN,Iosifidis T,et al.Reduced transforming growth factor beta1 (TGF-beta1) in the repair of airway epithelial cells of children with asthma[J].Respirology,2016,21(8):704-707.
[8] Chuang CY,Chen TG,Tai YT,et al.Toll-like receptor 2-mediated sequential activation of MyD88 and MAPKs contributes to lipopolysaccharide-induced sp-a gene expression in human alveolar epithelial cells[J].Immunobiology,2011,216(6):707-714.
[9] Yang C,Du W,Yang D.Inhibition of green tea polyphenol EGCG((-)-epigallocatechin-3-gallate) on the proliferation of gastric cancer cells by suppressing canonical wnt/beta-catenin signalling pathway[J].Int J Food Sci Nutr,2016,67(7):1-10.
[10] Lin YL,Chen SH,Wang JY.Critical role of IL-6 in dendritic cell-induced allergic inflammation of asthma[J].J Mol Med (Berl),2016,94(1):51-59.
[11] Huang H,Nie W,Qian J,et al.Effects of TNF-alpha polymorphisms on asthma risk:a systematic review and meta-analysis[J].J Investig Allergol Clin Immunol,2014,24(6):406-417.
[12] 石惠玲,刘杰波,芦爱萍.PI3K、NF-κB和STAT1在支气管哮喘儿童外周血单个核细胞的表达和意义[J].中国当代儿科杂志,2016,18(7):614-617.
[13] Baba Y,Sonoda JI,Hayashi S,et al.Reduction of oxidative stress in liver cancer patients by oral green tea polyphenol tablets during hepatic arterial infusion chemotherapy[J].Exp Ther Med,2012,4(3):452-458.
[14] Wu KJ,Hsieh MT,Wu CR,et al.Green Tea Extract Ameliorates Learning and Memory Deficits in Ischemic Rats via Its Active Component Polyphenol Epigallocatechin-3-gallate by Modulation of Oxidative Stress and Neuroinflammation[J].Evid Based Complement Alternat Med,2012,2012(1741-427X):214-215.
PDF(1288 KB)
