Effects and potential mechanisms of congenital lead exposure on interdigestie migrating motor complex changes of stomach,duodenum and jejunum in offspring rats

ZHOU Jian-ping; WANG Fan; WANG Jing-jing; JIANG Yong-sheng; GENG Yan; WANG Xue-ying; ZHONG Bo

doi:10.11852/zgetbjzz2017-25-09-11

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Chinese Journal of Child Health Care ›› 2017, Vol. 25 ›› Issue (9) : 900-899. DOI: 10.11852/zgetbjzz2017-25-09-11

Effects and potential mechanisms of congenital lead exposure on interdigestie migrating motor complex changes of stomach,duodenum and jejunum in offspring rats

ZHOU Jian-ping¹,WANG Fan²,WANG Jing-jing¹,JIANG Yong-sheng¹,GENG Yan¹,WANG Xue-ying¹,ZHONG Bo¹

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Abstract

Objective To analyze the effect and potential mechanisms of congenital lead poisoning on interdigestove migrating motor complex (IMMC) changes of stomach,duodenum and jejunum in offspring rats.Method The animal model of congenital lead exposure was established with pregnant rats drinking freely 0.1% (low-dose group,LG) and 0.2% (high-dose group,HG) lead acetate solution.The normal control group (NG) was also established.IMMC changes of stomach,duodenum and jejunum of male offspring rats were observed in technique of electrophysiological monitor.The methods of immunohistochemistry and radioimmunoassay were also applied to elucidate the potential mechanism of gastrointestinal (GI) motility disorders in the plasma levels and GI tissue expressions of CCK-8 and MTL.Results Compared with group NG,the periods and amplitudes of GI IMMC Ⅲ phase increased (P<0.01) in the offspring rats with lead exposure in embryo period.Furthermore,the increase of CCK-8 expressions and decreasing MTL expressions in gastric antrum mucosa (P<0.01),increasing expressions of CCK-8 and MTL in proximal duodenal mucosa (P<0.01),and as well as increasing levels of plasma CCK and MTL (P<0.01) were observed in groups LG and HG.The differences among these three groups were of statistical significance (P<0.01).Conclusions The abnormal changes of GI motility are observed in offspring rats with lead exposure in embryo period.It is possible that the different expressions or distribution of CCK and MTL in the peripheral tissue and blood are the important mechanisms for these pathological changes.

Key words

interdigestive migrating motor complex / brain-gut peptide / embryonic period / lead exposure / rat

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ZHOU Jian-ping,WANG Fan,WANG Jing-jing,JIANG Yong-sheng,GENG Yan,WANG Xue-ying,ZHONG Bo. Effects and potential mechanisms of congenital lead exposure on interdigestie migrating motor complex changes of stomach,duodenum and jejunum in offspring rats[J]. Chinese Journal of Child Health Care. 2017, 25(9): 900-899 https://doi.org/10.11852/zgetbjzz2017-25-09-11

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