Objective To study the physiological indexes of children receiving cardiopulmonary exercise testing(CPET)in order to establish the normal CPET reference value in Chinese children. Methods Four hundred and fifty healthy children underwent the CPET with the standard scheme using the cardiopulmonary testing system.There were fifty children per age,and the gender ratio was 1∶1.The indexes included oxygen uptake at anaerobic threshold (VO₂@AT),peak oxygen uptake (VO₂ peak),peak oxygen pulse (peak VO₂/HR),minimum of respiratory minute volume to CO₂ production (VE/VCO₂ minimum),slope of respiratory minute volume to CO₂ production (VE/VCO₂ slope),slope of oxygen uptake efficiency (OUES),peak tolerance power (Pmax),peak heart rate (HRmax) and maximal minute ventilation(VEmax). Results The VO₂ peak of boys was:4~6 years old:(816.36±152.03)ml/min;7~9 years old:(1 132.19±261.6)ml/min;10~12 years old:(1 418.99±270.2)ml/min respectively.The VO₂ peak of girls was:4~6:(700.36±139.77)ml/min;7~9 years old:(1 004.19±198.5)ml/min;10~12:(1 264.97±230.2)ml/min.VO₂@AT,VO₂ peak,Peak VO₂/HR,OUES,Pmax and VEmax all increased with age (P<0.001).VE/VCO₂ minimum and VE/VCO₂ slope decreased with age(P<0.001).The prediction equations for the above indexes were established by parameters of anthropometry.Peak VO₂/HR=-2.757+0.148×age (y) +0.049 × height (cm) +0.040× weight (kg) +0.892 × gender ("boys" =1,"girls" =0). Conclusions This study is to provide CPET medical reference value based on a large sample of Chinese children.The study can be used under the circumstances that there is no pediatric medical reference of CPET in China.