Research of the  dose  of  bifidobacterium  in the application in neonatal necrotizing enterocolitis in newborn rats

YANG Guo-peng; ZHU Ming-zhe; WANG Fang-fang; LAO Cheng-jun

doi:10.11852/zgetbjzz2017-25-08-10

PDF(614 KB)

Chinese Journal of Child Health Care ›› 2017, Vol. 25 ›› Issue (8) : 789-792. DOI: 10.11852/zgetbjzz2017-25-08-10

Research of the dose of bifidobacterium in the application in neonatal necrotizing enterocolitis in newborn rats

YANG Guo-peng¹,ZHU Ming-zhe²,WANG Fang-fang¹,LAO Cheng-jun¹

Author information +

History +

Abstract

Objective To study the dose of bifidobacteria in preventing neonatal necrotizing enterocolitis (NEC) in newborn rats,and to provide theoretical for clinical rational drug use. Methods A total of 40 SD rats were divided into 5 groups randomly:A,the normal group;B,NEC model group,give them lavage with normal saline daily while making model;C,NEC model group,establish the model and give 0.4 mg/g of duplex butyric acid bacteria living bacterium(Its main effective components is bifidobacterium)to fill the stomach daily;D,NEC model group,established the model and give 4 mg/g of duplex butyric acid bacteria living bacterium to fill the stomach daily;E,NEC model group,established the model and give 20 mg/g duplex butyric acid bacteria living bacterium to fill the stomach daily;Weighed their body weights and recorded everyday.Three days later,to fast one day.Put all the newborn mice to death.Anatomy all the rats and detect the contents of TNF-α and contrast them,observe the changes of their intestinal tissue and compared them;Observe the pathological changes of the intestinal tissue with HE staining. Results The average changes of body weight were A>D>E>C>B.There was no significance between group D and group E and no significance between group B and group C.Statistical significance existed among other groups.The mean scores of their HE staining pathology were B>D>C>E>A,there was statistical significance between A and other groups only.The averages of their TNF-α were group B>group C>group D>group E>group A.Based on the analysis of the above data,there was no significance between group D and group E and no significance between group B and group C.Statistical significance existed among other groups. Conclusion The optimal dose of duplex butyric acid bacteria living bacterium in neonatal necrotizing enterocolitis mice model may be 4 to 20 mg/g.The equivalent dose of bifidobacterium is 4×10³ CFU to 20×10³CFU.

Key words

neonatal necrotizing enterocolitis / bifidobacterium / dose / newborn rats

Cite this article

EndNote

Ris (Procite)

Bibtex

Download Citations

YANG Guo-peng,ZHU Ming-zhe,WANG Fang-fang,LAO Cheng-jun. Research of the dose of bifidobacterium in the application in neonatal necrotizing enterocolitis in newborn rats[J]. Chinese Journal of Child Health Care. 2017, 25(8): 789-792 https://doi.org/10.11852/zgetbjzz2017-25-08-10

References

[1] Thyoka M,de Coppi P,Eaton S,et al.Advanced necrotizing enterocolitis part 1:mortality[J].Eur J Pediatr Surg,2012,22(1):8-12.
[2] Murgas Torrazza R,Li N,Young C,et al.Pilot study using proteomics to identify predictive biomarkers of necrotizing enterocolitis from buccal swabs in very low birth weight infants[J].Neonatology,2013,104(3):234-242.
[3] Kastenberg ZJ,Sylvester KG.The surgical management of necrotizing enterocolitis[J].Clin Perinatol,2013,40(1):135-148.
[4] Rowland KJ,Choi PM,Warner BW.Role of growth factors in intestinal regeneration and repair in necrotizing enteroeolitis[J].Seminars in Pediatric Surgery,2013,22(2):101-111.
[5] 茹彩旺,吴福根,秦涛,等.双歧杆菌预防早产儿坏死性小肠结肠炎的疗效观察阴[J].中国微生态学杂志,2013,25(1):45-47.
[6] Tran L,Ferris M,Norori J,et a1.Necrotizing enterocolitis and cytomegalovirus infection in a premature infant[J].Pediatrics,2013,131(1):e318-e322.
[7] De Plaen IG.Inflammatory signaling in neerotizing enterocolitis[J].Clinicsin Perinatology,2013,40(1):109-124.
[8] 易小莲,张阿宏,严彩霞,等.新牛儿坏死性小肠结肠炎发病机制研究进展[J].中国新生儿科杂志,2011,26(2):130-132.
[9] Neu J,Walker WA.Necrotizing enterocolitis[J].N Engl J Med,2011,364(3):255-264.
[10] Sheng Q,Lv z,Cai W,et al.Protective effects of hydrogenrich saline on necrotizing enterocolitis in neonatal rats[J].J Pediatr Surg,2013,48(8):1697-1706.
[11] Bhatia AM,Stoll BJ,Cismowski MJ,et al.Cytokine levels in the preterrn infant with neonatal intestinal injury[J].Am J Perinatol,2013,31(6):489-496.
[12] Hogberg N,Stenback A,Carlsson PO,et al.Genes regulating tight junctions and cell adhesion are altered in early experimental necrotizing enterocolitis[J].J Pediatr Surg,2013,48(11):2308-2312.
[13] Manzoni P,Lista G,Gallo E,et al.Routine Lactobacillus rhamnosus GG administration in VLBW infants:a retrospective,6-year cohort study[J].Early Hum Dev,2011,87:s35-38.
[14] Deshpande GC,Rao SC,Keil AD,et al.Evidence-based guidelines for use of probioties in preterm neonates[J].BMC Med,2011,9:92-104.
[15] Mirpuri J,Sotnikov I,Myers L,et al.Lactobaeillus rhamnosus(LGG)regulates IL-10 signaling in the developing mufine colon through up regulation of the IL-10R2 receptor subunit[J].PLoS One,2012,7:51-55.
[16] Zhou W,Li W,Zheng XH,et al.Glutamine downregulates TLR-2 and TLR-4 expression and protects intestinal tract in preterm neonatal rats with neerotizing enterocolitis[J].J Pediatr Surg,2014,49:1057-1063.
[17] Lu P,Sodhi CP,Jia H,et al.Animal models of gastrointestinal and liver diseases.Animal models of necrotizing enterocolitis:pathophysiology,translational relevance,and challenges[J].Am J Physiol Gastrointest Liver Physiol,2014,306:G917-928.
[18] 吴文燊,王艳丽,温婉文,等.双歧杆菌对新生大鼠坏死性小肠结肠炎模型的保护作用[J].中国新生儿科杂志,2016,31(3):212-216.
[19] 苏衡,吕回,周伟,等.双歧杆菌对坏死性小肠结肠炎新生大鼠肠道保护作用的机制[J].中华围产医学杂志,2015,18(4):290-295.
[20] 李美雪,周伟,吕回,等.脂多糖联合人工喂养诱导新生大鼠坏死性小肠结肠炎模型的建立与评价[J].发育医学电子杂志,2014,2(1):21-27.
[21] 富琴琴,林梅芳,邓庆先,等.谷氨酰胺对新生大鼠坏死性小肠结肠炎肠道组织Caspase3及增殖细胞核抗原表达的影响[J].中国新生儿科杂志,2016,31(2):139-144.