Objective To investigate the clinical analysis of lower gastrointestinal bleeding caused by cow's milk protein allergy in infants,and to provide a basis for early diagnosis and effective treatment of lower gastrointestinal bleeding caused by cow's milk protein allergy in infants. Method The clinical data of 60 infants with lower gastrointestinal bleeding caused by cow's milk protein allergy in Shenzhen Children's Hospital from May 2016 to January 2014 were analyzed retrospectively. Results Most cow's milk protein allergy caused lower gastrointestinal bleeding was in infants with the age of 1~6 months(68.33%),with no significant gender difference in age distribution(χ²=1.371,P=0.242),25 cases of formula-feeding infants (41.67%);Clinical manifestation was blood in stool suddenly in 46 cases (76.67%);Experimental examination of IgG antibody increased in 48 cases (80%);Electronic colonoscopy revealed multiple nodular hyperplasia (81.33%);Pathological examination showed chronic nonspecific inflammation,and the intrinsic layer of eosinophils increased; After avoid milk protein treatment:cure rate 2 weeks' was significantly increased than that of one-week.The difference was statistically significant (P<0.05);Between 4 weeks' and two weeks' cure rate,the difference was not statistically significant (P>0.05). Conclusions Milk protein allergy caused lower gastrointestinal bleeding,mainly are infants with the age of 1~6 months with formula-feeding infants,elevated IgG antibody maybe involve in the pathogenesis of infant lower gastrointestinal bleeding caused by cow's milk protein allergy.Avoidance of milk protein of 2~4 weeks,the lower gastrointestinal bleeding can not be alleviated,patients need to consider the cause of eosinophilic colitis.