Objective To explore the role for connective tissue growth factor(CTGF) in the lung development by dynamic observation of morphology and the expression of CTGF in lungs of rat pups exposed to antenatal inflammation. Methods Pregnant Wistar rats were randomly divided into experimental group and control group.Pregnant rats of both groups were intraperitoneal injected with either lipopolysaccharide ( LPS)2.5 mg/kg or the same volume of normal saline on embryonic day 19 and 20,respectively,and were allowed to term deliver.On postnatal days 1,3,7 and 14 (D1,D3,D7 and D14),eight pups of each group were killed by 10% chloral hydrate (1 ml/kg) and lungs were collected.Left lungs of each group were used to assess lung histological changes after hematoxylin and eosin(HE) staining by measuring the mean numbers of alveolar,the mean ratio of alveolar surface area to per tissue and the mean thickness of alveolar septum.Right lungs of each group were used to measure the expression of CTGF mRNA and protein by Real-time polymerase chain reaction (Real-time PCR) and Western blot. Results 1)With the increasing of postnatal days,the mean numbers of alveolar and the mean ratio of alveolar surface area to per tissue in both groups increased,the mean thickness of alveolar septum got thinner.On D1,D3,D7 and D14,the mean alveolar numbers of the experimental group were significantly less than those of the control group (P<0.05 or <0.01).On D1,D3 and D7,the mean ratios of alveolar surface area to per tissue were significantly larger than those of control group(P<0.01).On D1 and D3,the alveolar septum thickness was significantly thinner than that in control group(P=0.000).2)On D1,D3,andD7,the expression of CTGF mRNA of the experimental group were significantly higher than those of the control group(P<0.05 or <0.01).3)On D1,D3,D7 and D14,the expression of CTGF protein of the experimental group were significantly higher than those of the control group(P all<0.05). Conclusion Antenatal infection can up-regulate expression of CTGF,which may assiociate with disorders of lung development.
Key words
antenatal infection /
lung development /
connective tissue growth factor /
rat /
bronchopulmonary dysplasia
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