Expression of synaptic associated proteins in the prefrontal cortex of autism rats

CHEN Yun-hua; GOU Yun; ZHOU Bo; LI Hong-wei; LIU Fen; TONG Xue-tao; WEN Min

doi:10.11852/zgetbjzz2017-25-05-11

PDF(695 KB)

Chinese Journal of Child Health Care ›› 2017, Vol. 25 ›› Issue (5) : 470-473. DOI: 10.11852/zgetbjzz2017-25-05-11

Orignal Article

Expression of synaptic associated proteins in the prefrontal cortex of autism rats

CHEN Yun-hua¹, GOU Yun¹, ZHOU Bo², LI Hong-wei¹, LIU Fen³, TONG Xue-tao³, WEN Min¹

Author information +

History +

Abstract

Objective To observe the change of the synapse associated protein synaptophysin (SYN),postsynappptic density protein-95 (PSD-95),gephyrin in the prefrontal cortex of the rat model of autism and explore the role of synapse associated protein in the pathogenesis of autism. Method sThe autism model group was obtained in the offspring female Wister rats those received a single intraperitoneal injection of sodium valproate (600mg/kg) on the 12.5th pregnancy day.Pregnant rats received the intraperitoneal injection of saline,whose offspring were defined as normal group.To confirm whether the animal model were successfully established,the model group were compared the oblique board test,opening eyes test and three-chamber sociability test with control group.Western blotting was used to detected the expressed level of SYN,PSD-95,gephyrin in prefrontal cortex of model group and control group in the 42th day after birth. Results 1)The animal model of autism was successfully established.Compared with control rats,the autistic ones were slower to grow,poorer social interaction ability,and the preference for fresh things was disorder,the differences were statistically significant(all P<0.05);2)Compared with control group,the model group significantly increased the expression level of SYN and PSD-95 (P<0.05) and decreased the expression level of gephyrin(P<0.05) in prefrontal cortex. Conclusion The expression of synaptic associated proteins in the prefrontal cortex of autism rats is abnormal.

Key words

autism / synaptophysin / postsynappptic density protein-95 / gephyrin / prefrontal cortex

Cite this article

EndNote

Ris (Procite)

Bibtex

Download Citations

CHEN Yun-hua, GOU Yun, ZHOU Bo, LI Hong-wei, LIU Fen, TONG Xue-tao, WEN Min. Expression of synaptic associated proteins in the prefrontal cortex of autism rats[J]. Chinese Journal of Child Health Care. 2017, 25(5): 470-473 https://doi.org/10.11852/zgetbjzz2017-25-05-11

References

[1] Buckley AW,Holmes GL.Epilepsy and autism[J].Cold Spring Harb Perspect Med,2016,6(4).doi:10.1101/cshperapect.a022749.
[2] Lai MC,Lombardo MV,Baron-Cohen S.Autism[J].Lancet,2014,383(9920):896-910.
[3] 武丽杰.我国孤独症谱系障碍流行病学现状及趋势[J].中国实用儿科杂志,2013,28(8):571-576.
[4] Curatolo P,Ben-Ari Y,Bozzi Y,et al.Synapses as therapeutic targets for autism spectrum disorders:an international symposium held in pavia on july 4th,2014[J].Front Cell Neurosci,2014,8:309.
[5] 邵世怡,陈琳,蒋权,等.基于突触功能异常的自闭症发病机制研究进展[J].中国现代应用药学,2016,33(4):501-508.
[6] Schneider T,Przewlocki R.Behavioral alterations in rats prenatally exposed to valproic acid:animal model of autism[J].Neuropsychopharmacology,2005,30(1):80-89.
[7] Abrahams BS,Geschwind DH.Advances in autism genetics:on the threshold of a new neurobiology[J].Nat Rev Genet,2008,9(5):341-355.
[8] Rasalam AD,Hailey H,Williams JH,et al.Characteristics of fetal anticonvulsant syndrome associated autistic disorder[J].Dev Med Child Neurol,2005,47(8):551-555.
[9] Stromland K,Nordin V,Miller M,et al.Autism in thalidomide embryopathy:a population study[J].Dev Med Child Neurol,1994,36(4):351-356.
[10] Kim KC,Kim P,Go HS,et al.The critical period of valproate exposure to induce autistic symptoms in Sprague-Dawley rats[J].Toxicol Lett,2011,201(2):137-142.
[11] Rodier PM,Ingram JL,Tisdale B,et al.Linking etiologies in humans and animal models:studies of autism[J].Reprod Toxicol,1997,11(2-3):417-422.
[12] Yang EJ,Ahn S,Lee K,et al.Early Behavioral abnormalities and perinatal alterations of PTEN/AKT pathway in valproic acid autism model mice[J].PLoS One,2016,11(4):e153298.
[13] Cheaha D,Bumrungsri S,Chatpun S,et al.Characterization of in utero valproic acid mouse model of autism by local field potential in the hippocampus and the olfactory bulb[J].Neurosci Res,2015,98:28-34.
[14] 文敏,赵晶,鲍星星,等.孤独症大鼠前额叶皮质神经元凋亡相关蛋白的表达[J].山东大学学报:医学版,2014,52(2):49-51.
[15] Gatto C L,Broadie K.Genetic controls balancing excitatory and inhibitory synaptogenesis in neurodevelopmental disorder models[J].Front Synaptic Neurosci,2010,2:4.
[16] 刘轲,鲁红伟,冯静静,等.脑脉通联合骨髓间充质干细胞移植对脑缺血再灌注大鼠神经细胞中SYN-38和PSD-95表达变化的影响[J].时珍国医国药,2015,26(5):1048-1051.
[17] Alvarez VA,Sabatini BL.Anatomical and physiological plasticity of dendritic spines[J].Annu Rev Neurosci,2007,30(1):79-97.
[18] Courchesne E,Mouton PR,Calhoun ME,et al.Neuron number and size in prefrontal cortex of children with autism[J].JAMA,2011,306(18):2001-2010.
[19] 刘夫军,袁向山,刘静,等.小胶质细胞在VPA孤独症模型大鼠脑增龄过程中的变化[J].神经解剖学杂志,2015,31(3):275-281.
[20] Xu W.PSD-95-like membrane associated guanylate kinases (PSD-MAGUKs) and synaptic plasticity[J].Curr Opin Neurobiol,2011,21(2):306-312.
[21] Petrini EM,Ravasenga T,Hausrat TJ,et al.Synaptic recruitment of gephyrin regulates surface GABAA receptor dynamics for the expression of inhibitory LTP[J].Nat Commun,2014,5:3921.
[22] Choii G,Ko J.Gephyrin:a central GABAergic synapse organizer[J].Exp Mol Med,2015,47(2):e158.