Objective To explore the effects of CTRP9 on proliferation of airway smooth muscle cells (ASMCs) and airway inflammation in infant asthmatic mice. Methods Elisa kits were used for detecting the contents of CTRP9 in serum from the normal and asthmatic children and mice respectively.ASMCs of asthmatic mice were isolated and cultured followed by transfection with pcDNA3.1-CTRP9,MTT were used to determine the level of cells proliferation and elisa kits were used again to measure the levels of TNF-α and IL-6.Besides,the levels of TLR4,NF-κB p65 and phosphorylation of NF-κB p65 were detected by Western blot analyses.Asthmatic mice were transfected with pcDNA3.1-CTRP9 and observed the infiltration of inflammatory cells in lung tissues by HE staining.The number of eosinophils,neutrophils and macrophages in BALF were counted under light microscope.Finally,the levels of TNF-α,IL-6,TLR4 and NF-κB in lung of mice were measured again as above methods. Results The asthmatic children and mice showed lower CTRP9 content and mRNA expression in serum compared to control group.After transfection ASMCs with pcDNA3.1-CTRP9,the cells proliferation,inflammatory cytokines secretion and TLR4/NF-κB pathway were all inhibited obviously.Moreover,overexpression of CTRP9 in asthmatic mice suppressed the infiltration of inflammatory cells in lung tissues,the number of inflammatory cells,as well as the inflammatory cytokines secreting and activation of TLR4/NF-κB. Conclusion CTRP9 inhibits the proliferation of airway smooth muscle cells and attenuates inflammation in asthmatic mice.
Key words
airway smooth muscle cells /
inflammation /
TLR4/NF-κB /
asthma /
CTRP9
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