Objective To investigate the effect of strengthening of breastfeeding on the prevention of infection in very low birth weight infants. Methods 80 preterm infants whose birth weight between 1 000 g and 1 500 g were involved in this study.According to the feeding mode,the infants were divided into two groups.The reinforcement of breastfeeding group (experimental group,n=40,The proportion of breast milk during hospitalization accounted for more than 75% of the total feeding,breast milk was from the children's own mother.When the feeding amount was more than 80 ml/ (kg·d),human milk fortifier was added).Formula milk feeding group (control group,n=40,all preterm infants were fed with formula during hospitalization).The time of reaching total enteral feeding,feeding intolerance,nosocomial infection,and the incidence rate of neonatal necrotizing enterocolitisin of the two groups were compared. Results Two groups had no diffences in gestational age or gender or birth weight(P>0.05).The time of reaching total enteral feeding of experimental group was(15.22±4.37)d,while the control group was (17.38±5.44)d.Statistically significant difference was demonstrated in time of reaching total enteral feeding between the two groups(P<0.05).The cases of feeding intolerance in the experimental group were 7,and in the control group were 15,statistically significant difference was demonstrated in feeding intolerance between the two groups(P<0.05).The nosocomial infection cases in the experimental group were 2,and in the control group were 7,statistically significant difference was demonstrated in nosocomial infection between the two groups(P<0.05).The incidence rate of neonatal necrotizing enterocolitis of the two groups had no significant differences (P>0.05). Conclusions Strengthening of breastfeeding in very low birth weight infants can shorten the time of reaching total enteral feeding,decrease the incidence of feeding intolerance and nosocomial infection,meanwhile do not influence the occurrence of neonatal necrotizing enterocolitis.