Effects of budesonide on airway remodeling through regulating the expression of ORMDL3 and MMP-9 in chronic asthma mouse model

YU Jia-chen; YU Fei; SUN Yan

doi:10.11852/zgetbjzz2017-25-02-09

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Chinese Journal of Child Health Care ›› 2017, Vol. 25 ›› Issue (2) : 139-142. DOI: 10.11852/zgetbjzz2017-25-02-09

Effects of budesonide on airway remodeling through regulating the expression of ORMDL3 and MMP-9 in chronic asthma mouse model

YU Jia-chen¹,YU Fei²,SUN Yan³

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Abstract

Objective To investigate the effect of budesonide on the expression of orosomucoid1-like3(ORMDL3),matrix metalloproteinases-9(MMP-9) and understand the mechanism of budesonide in inhibition of airway remodeling in chronic asthma mice. Method Mice were divided into three groups,including the asthmatic model group,the budesonide group and the control group.The asthmatic model were established by administrating OVA and aluminum hydroxide.The BUD group were administered with aerosol budesonide (100 μg/kg) before OVA challenge from day 21.The control group were sensitized and challenged with PBS instead.Using HE staining and Masson staining to assess the extent of bronchial inflammation and collagen deposition in each group.The expression of ORMDL3 and MMP-9 were examined in absence and presence of treatment via immunohistochemistry,RT-PCR and Western blotting in 12 weeks. Results The asthma group showed more inflammatory responses and airway remodeling compared with the control group and the BUD group.Administration of BUD decreased the severity of pathological changes but not eliminated these changes.Data proved that both ORMDL3 and MMP-9 increased significantly in asthma group while increased slightly in BUD group.Besides,the increase of ORMDL3 had a significant correlation with MMP-9 level.And content of ORMDL3,MMP-9 had positive correlation with the thickness of airway. Conclusion Administration of BUD may down-regulate the expression of ORMDL3 and MMP-9 to reduce airway remodeling.

Key words

budesonide / orosomucoid 1-like 3 / matrix metalloprpteinases-9 / airway remolding / chronic asthma

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YU Jia-chen,YU Fei,SUN Yan. Effects of budesonide on airway remodeling through regulating the expression of ORMDL3 and MMP-9 in chronic asthma mouse model[J]. Chinese Journal of Child Health Care. 2017, 25(2): 139-142 https://doi.org/10.11852/zgetbjzz2017-25-02-09

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