Effects of lithium chloride on the activity of PI3K/Akt signaling pathway in hypoxic neural stem cells

CHEN Chang-chun; ZHOU Qin; YAN Yan; WANG Qi; ZHANG Xiao-yuan; YIN Xiao-juan

doi:10.11852/zgetbjzz2017-25-02-08

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Chinese Journal of Child Health Care ›› 2017, Vol. 25 ›› Issue (2) : 135-138. DOI: 10.11852/zgetbjzz2017-25-02-08

Effects of lithium chloride on the activity of PI3K/Akt signaling pathway in hypoxic neural stem cells

CHEN Chang-chun^1,2,ZHOU Qin²,YAN Yan^1,3,WANG Qi^1,4,ZHANG Xiao-yuan^1,5,YIN Xiao-juan¹

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Abstract

Objective To investigate the effect of lithium chloride on PI3K/Akt signaling pathway in hypoxic neural stem cells (NSCs). Methods The models of hypoxic NSCs were established by culturing and identify NSCs isolated from neonatal SD rat.The NSCs of normal control group was cultured in serum-free medium.The culture medium of normal saline intervention group was immediately added normal saline,and lithium chloride intervention group was immediately added different concentration of lithium chloride.Akt /P-Akt signaling pathway markers of NSCs were detected by immunohistochemistry. Results The amount of NSCs decreased and the characteristics of the NSCs was abnormal in shape,such as low refraction,swollen perikarya or even membranolysis.The number of dead cells of NSCs in hypoxic group increased significantly (P<0.05).The activeness of NSCs in hypoxic group decreased significantly (P<0.05).Compared with hypoxic group,normal saline intervention group and 5 mM lithium chloride intervention group,P-Akt expression level of hypoxic NSCs in 1 mM lithium chloride intervention group increased significantly,while decreased significantly compared with 3 mM lithium chloride intervention group.Compared with 1 mM and 5 mM lithium chloride intervention group,P-Akt expression level of hypoxic NSCs in 3 mM lithium chloride intervention group increased significantly.Compared with other groups,the number of hypoxic NSCs positive expressing P-Akt in 3 mM lithium chloride group increased significantly (P<0.05). Conclusions Lithium chloride activates PI3K/Akt signaling pathway in hypoxic NSCs.

Key words

neural stem cells / hypoxia model / signaling pathway / lithium chloride

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CHEN Chang-chun,ZHOU Qin,YAN Yan,WANG Qi,ZHANG Xiao-yuan,YIN Xiao-juan. Effects of lithium chloride on the activity of PI3K/Akt signaling pathway in hypoxic neural stem cells[J]. Chinese Journal of Child Health Care. 2017, 25(2): 135-138 https://doi.org/10.11852/zgetbjzz2017-25-02-08

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