Objective To study the correlation of newborn cord blood 25(OH)D and total IgE levels. Method Cord blood 25(OH)D and total IgE levels were measured in 185 newborns.The health status of pregnant women and history of newborn birth were recorded by questionnaires;The status of offspring allergy was evaluated at approximately 1 year. Results The average and median value of 25(OH)D levels were (49.63±16.56) nmol/L and 49.50nmol/L;The average value of IgE level was (513.85±116.54) U/L;38 infants experienced allergy at follow-up;Cord blood 25(OH)D level in allergic infants was (38.88±12.33) nmol/L,and (52.41±16.41) nmol/L in non-allergic infants,there was significant difference between them (P<0.05);Cord blood total IgE level in allergic infants was higher than non-allergic infants (P<0.05);Family history of allergy and cord blood 25(OH)D level had association with cord blood total IgE level by Univariate analysis;Cord blood 25(OH)D level was inversely associated with total IgE level (r²=0.576,P<0.05). Conclusion Cord blood 25(OH)D level has an negative association with total IgE and reduced vitamin D level in cord blood would be a risk factor for the development of allergy in the first year of life.