Objective To study the human milk resource peptide in the role of resistance of neonatal infection. Methods The content of β-Casein-15 in human milk was mesured by mass spectrometry technology.The biologic characteristics of β-Casein-15 peptide were predicted by Uniprot,SABLE,ProtParam tool and other online databases. Results The online databases showed that aliphatic index,grand average of hydropathicity and instability coefficient of β-Casein-15 were 122.33,-0.387 and 36.29,respectively.It indicated that β-Casein-15 was a hydrophobic stable peptide.And then,the content of β-Casein-15 was significantly decreased in premature compared to full term infants,detected by mass spectrometry technology.Moreover,the bacteriostasis experiment was found β-Casein-15 peptide in e.coli,enterocolitis bacteria and staphylococcus aureus had obvious bacteriostatic action. Conclusion These results indicate β-Casein-15 may play a critial role in promoting neonatal anti-infection.
Key words
human milk /
β-Casein-15 peptide /
bacteriostatic
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
References
[1] 倪国华,张璟,郑风田.中国肥胖流行的现状与趋势[J].中国食物与营养,2013,19(10):70-74.
[2] 刘平,李宗军,许爱清.胃肠道微生态系统及其功能研究[J].中国微生态学杂志,2010,22(3):277-278,281.
[3] Hayakawa E,Menschaert G,De Bock PJ,et al.Improving the identification rate of endogenous peptides using electron transfer dissociation and collision-induced dissociation[J].J Proteome Res,2013,12(12):5410-5421.
[4] Choi YY.Necrotizing enterocolitis in newborns:update in pathophysiology and newly emerging therapeutic strategies[J].Korean J Pediatr,2014,57(12):505-513.
[5] Sanchez A,Calderon E,Castanon-Alonso SL,et al.In vitro determination of the short-chain synthetic peptide RP13 antimicrobial activity[J].Rev Invest Clin,2014,66(6):527-533.
[6] 李冠楠,夏雪娟,隆耀航,等.抗菌肽的研究进展及其应用[J].动物营养学报,2014,26(1):17-25.
[7] 李振国,胡芳,王素芳.170例新生儿肺炎患者临床抗生素的使用情况分析[J].抗感染药学,2015,12(5):671-672.
[8] Hirsiger P,Malik J,Kummerlen D,et al.Risk factors for the oral use of antibiotics and treatment incidence of weaners in Switzerland[J].Schweiz Arch Tierheilkd,2015,157(12):682-688.
[9] 黎观红,洪智敏,贾永杰,等.抗菌肽的抗菌作用及其机制[J].动物营养学报,2011,23(4):546-555.
PDF(592 KB)
