Objective To explore the clinical value of wide angle digital retinal imaging system (RetCam3) in the screening of neonatal,and follow up analysis of the prognosis of the patients. Method Retrospective analysis of clinical data of neonates with RetCam3 in our hospital from January 2013 to December 2015 in the department of obstetrics and neonatal intensive care unit. Results After screening the 15 750 cases,5 196 cases (32.99%) were identified with fundus lesions,including 4 312 cases with retinal hemorrhage (RH),312 cases with retinal white changes,186 cases with retinal pigmentation,132 cases with class of retinopathy of prematurity in full-term neonates under changes,64 cases with abnormal retinal blood vessels,56 cases with retinopathy of prematurity (ROP),40 cases with choroidal defect,27 cases with retinal jaundice,17 cases with optic nerve dysplasia,14 cases with vitreous hemorrhage,12 cases with persistent hyperplastic primary vitreous (PHPV),10 cases with preretinal hemorrhage,6 cases with macular defects,4 cases with albinotic fundus,PHPV1 cases with morning glory syndrome,1 case with retinoblastoma (RB),1 case with incontinentia pigmenti (IP),1 case with advanced cup/disc ratio. Conclusion The wide angle digital retinal imaging system can be used to discover the ocular fundus diseases of newborn in time,and provide the basis for clinical real-time and effective guidance and intervention.