Objective To investigate the effect of lipid metabolism disorders in Sprague-Dawley (SD) rats on fetal rat myocardial cell. Methods Pregnant rats were randomly divided into A and B groups,each group had 10 cases.Group A was fed with ordinary feed,and group B with high fat feed,all were fed to pregnancy 21 days respectively.Blood samples were collected to determine blood lipid.Fetal rat's myocardial tissue was taken to observe under light microscope and electron microscope after dealing with. Results At 21 days of pregnancy,compared with group A,total cholesterol (TC) and low density lipoprotein cholesterol (LDL-C) of group B rats increased significantly (P=0.000),and high-density lipoprotein cholesterol (HDL-C) decreased obviously (P=0.001).Fetal rat's myocardial cell of two groups were observed with HE staining slice under light microscopy,cell structure of group B owes unclear with cell edema,cell nucleus shape was irregular.Fetal rat myocardial tissue ultrastructure observation was indicated:Group B cells organelles were decrease in the number and mitochondria crest fractured. Conclusion High-fat feeding of SD rats exists abnormal lipid metabolism during pregnancy (high cholesterol,high LDL-C and low HDL-C),the fetal rat myocardial cell has damage.
Key words
pregnancy /
lipid metabolism disorder /
myocardial cells /
SD rats
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