Objective To assess the efficacy of recombinant human growth hormone (rhGH) therapy on very late puberty girls whose bone aged 13~15.5 with idiopathic short stature (ISS) and discuss the necessary. Methods A total of 43 girls in very late puberty with ISS were divided into control group (n=15) and trial group (n=28) complying with the decision of the girls′ family.The participators in trial group were injected with rhGH 0.18~0.20 U/kg before sleep every night for 6 months,control group were observed for the same time without treatment.Bone age,growth velocity,prediction of adult height were compared between two groups.Totally 43 parents of very late puberty girls were surveyed by the self-administered questions of their own feelings about their own decision of using rhGH injection every day for a long time or not after 6 months. Results There were significant differences in growth velocity between before and after treatment in trial group (P<0.01).And after treatment with rhGH,there was significant difference of the growth velocity between two groups (P<0.01).After 6 months observation,the prediction of adult height was lower in control group,while the result was on the contrary in trial group.There were significant differences between after and before treatment of the value of the prediction of adult height between two groups (P<0.01).In the questionnaire after treatment,the curative effect and medical expense were acceptable for the parents in trial group.And the parents in control group were also calm with their decision of giving up rhGH treatment in the end. Conclusions The rhGH treatment for very late puberty girls can improve the growth velocity but can′t improve the prediction of adult height obviously.With good doctor-patient communications,the diagnosis and treatment process of these very late puberty girls with ISS leaded to a positive psychologic influence on their families both in trial group and control group after 6 months.It was suggested that weather very late puberty girls with ISS should be treated by rhGH should followed the patients′ opinion just on the basis of adequate understanding on their own condition.