Objective To evaluate the quality index of newborn hearing screening in Rugao and provide reference for the adjustment of the screening program. Method Retrospective analysis of newborn hearing screening was conducted in Rugao city during 2006-2013 to calculate the related quality indicators and make comparisons with the national quality standard. Results Newborn hearing screening rate in recent 8 years was 92.95%(79 802/85 857),exceeded the requirements of the national "screening program"(90%)(U=28.81,P＜0.05).The rate of the primary screening was 91.55%(73 060/79 802) and higher than the requirement of "listening test intervention guide" (90%)(U=14.60,P＜0.05).The rescreening rate was 95.85%(6 462/6 742),and higher than the requirement of "listening test intervention guide" (80%)(U=32.54,P＜0.05).Rescreening positive rate was 39.89%(2 578/6 462).The number of secondary screening positive referrals accounted for 2.19% of the total number of screening(1 748/79 802) and within the provisions of the "listening test intervention guide"(5%)(U=36.42,P＜0.05),referral rate was 67.80%(1 748/2 578).The diagnosis rate of hearing impairment was 0.99‰(79/79 802).Initial screening age of confirmed cases was (26.95±13.35) days(t=11.288,P＜0.05),secondary screening age was (58.16±15.50) days(t=9.268,P＜0.05) and lag behind the national "screening specification" requirements.Referral age was (96.44±26.84) days(t=1.720,P＞0.05),and in line with the national "screening specification" requirements. Conclusion The main problems of newborn hearing screening in Rugao are screening age,screening days behind the "screening specification" requirements,so there is need to adjust the hearing screening program in a timely manner and to carry out the investigation and research on related factors.