Influence of mesenchymal stem cell transplantation on brain-derived neurotrophic factor expression in rats with Tourette syndrome.

LIU Xiu-mei; WANG Xue-ming; YI Ming-ji

doi:10.11852/zgetbjzz2016-24-09-10

PDF(601 KB)

Chinese Journal of Child Health Care ›› 2016, Vol. 24 ›› Issue (9) : 928-930. DOI: 10.11852/zgetbjzz2016-24-09-10

Influence of mesenchymal stem cell transplantation on brain-derived neurotrophic factor expression in rats with Tourette syndrome.

LIU Xiu-mei¹, WANG Xue-ming², YI Ming-ji³

Author information +

History +

Abstract

Objective To investigate bone marrow mesenchymal stem cells (MSC) transplantation on brain-derived neurotrophic factor (BDNF) expression in the striatum of Tourette syndrome (TS) rat,and to explore the possible mechanism of MSC transplantation in the treatment of TS. Methods Seventy-two Wistar rats were randomly allocated to the control group and the two experimental groups,namely,TS rats+vehicle and TS rats+MSC.MSCs were co-cultured with 5-bromodeoxyuridine (BrdU) for 24 h for labeling prior to grafting.An autoimmune TS animal model was adopted in the present study.Rat MSCs were cultured and expanded using density gradient centrifugation in vitro,identified by flow cytometry and transplanted into the striatums of TS animal.BDNF level was detected by ELISA and RT-PCR techniques. Results Stereotypic behaviors reduced in TS rats 7 days after MSC transplantation into rat striatum.BDNF level in striatum of TS rat increased (P<0.05).After MSC transplantation,the BDNF level decreased in the striatum of TS rat. Conclusions Intrastriatal transplantation of MSCs can provide relief from the stereotypic behavior of TS.BDNF level reduces in TS rats after MSC transplantation.

Key words

bone marrow mesenchymal stem cells / transplantation / Tourette syndrome / brain-derived neurotrophic factor

Cite this article

EndNote

Ris (Procite)

Bibtex

Download Citations

LIU Xiu-mei, WANG Xue-ming, YI Ming-ji. Influence of mesenchymal stem cell transplantation on brain-derived neurotrophic factor expression in rats with Tourette syndrome.[J]. Chinese Journal of Child Health Care. 2016, 24(9): 928-930 https://doi.org/10.11852/zgetbjzz2016-24-09-10

References

[1] Tamara P.Tourette syndrome and other tic disorders of childhood[J].Handb Clin Neurol,2013,112:853-856.
[2] Liu X,Wang Y,Li D,et al.Transplantation of rat neural stem cells reduces stereotypic behaviors in rats after intrastriatal microinfusion of Tourette syndrome sera[J].Behav Brain Res,2008,186(1):84-90.
[3] Liu XM,Wang YW,Yi MJ.Effects of human mesenchymal stem cell transplantation in the bilateral corpus striatum in a rat model of Tourette's syndrome[J].Neural Regen Res.2010,5(17):1285-1290.
[4] Van Velthoven CT,Braccioli L,Willemen HL,et al.Therapeutic potential of genetically modified mesenchymal stem cells after neonatal hypoxic-ischemic brain damage[J].Mol Ther,2014,22(3):645-654.
[5] Taylor JL,Rajbhandari AK,Berridge KC,et al.Dopamine receptor modulation of repetitive grooming actions in the rat:potential relevance for Tourette syndrome[J].Brain Res,2010,1322:92-101.
[6] Malaty IA,Akbar U.Updates in medical and surgical therapies for Tourette syndrome[J].Curr Neurol Neurosci Rep,2014,14(7):458.
[7] Kordi-Tamandani DM,Sahranavard R,Torkamanzehi A.DNA methylation and expression profiles of the brain-derived neurotrophic factor (BDNF) and dopamine transporter (DAT1) genes in patients with schizophrenia[J].Mol Biol Rep,2012,39(12):10889-10893.
[8] Ferrini F,De Koninck Y.Microglia control neuronal network excitability via BDNF signaling[J].Neural Plast,2013,2013:429815
[9] Phinney DG,Isakova IA.Mesenchymal stem cells as cellular vectors for pediatric neurological disorders[J].Brain Res,2014,1573:92-107.
[10] Zhang F,Wang YY,Liu H,et al.Resveratrol produces neurotrophic effects on cultured dopaminergic neurons through prompting astroglial BDNF and GDNF release[J].Evid Based Complement Alternat Med,2012,2012:937605.doi:10.1155/2012/937605.
[11] Paulsen JS.Cognitive impairment in Huntington disease:diagnosis and treatment[J].Curr Neurol Neurosci Rep,2011,11(5):474-483.
[12] Niitsu T,Shirayama Y,Matsuzawa D,et al.Associations of serum brain-derived neurotrophic factor with cognitive impairments and negative symptoms in schizophrenia[J].Prog Neuropsychopharmacol Biol Psychiatry,2011,35(8):1836-1840.
[13] Mang CS,Campbell KL,Ross CJ,et al.Promoting neuroplasticity for motor rehabillitation after stroke:considering the effects of aerobic exercise and genetic variation on brain-derived neurotrophic factor[J].Phys Ther,2013,93(12):1707-1716.
[14] Shimada H,Makizako H,Doi T,et al.A large,cross-sectional observational study of serum BDNF,cognitive function,and mild cognitive impairment in the elderly[J].Front Aging Neurosci,2014,6:69.
[15] Li Y,Yui D,Luikart BW,et al.Conditional ablation of brain-derived neurotrophic factor-TrkB signaling impairs striatal neuron development[J].Proc Natl Acad Sci USA,2012,109(38):15491-15496.
[16] Martino D,Chiarotti F,Buttiglione M,et al.The relationship between group A streptococcal infections and Tourette syndrome:a study on a large service-based cohort[J].Dev Med Child Neurol,2011,53(10):951-957.
[17] Yeh TK,Hu CY,Yeh TC,et al.Association of polymorphisms in BDNF,MTHFR,and genes involved in the dopaminergic pathway with memory in a healthy Chinese population[J].Brain Cogn,2012,80(2):282-289.