Effect of vitamin D receptor expression in the thymus of filial generation mice after giving pregnant vitamin D.

WANG Zhi-hui; ZHAO Xia-xia; REN LI-hong.

doi:10.11852/zgetbjzz2016-24-07-11

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Chinese Journal of Child Health Care ›› 2016, Vol. 24 ›› Issue (7) : 712-714. DOI: 10.11852/zgetbjzz2016-24-07-11

Effect of vitamin D receptor expression in the thymus of filial generation mice after giving pregnant vitamin D.

WANG Zhi-hui,ZHAO Xia-xia,REN LI-hong.

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Abstract

Objective To detect the changes of vitamin D receptor (VDR) expression in the thymus of filial generation mice after giving pregnant mice different dosages of vitamin D supplementation. Methods Totally 18 female Wistar mice mates with 9 male Wistar mice at 5 pm everyday (the female/male radio was 2∶1).The vaginal secretions of the female mice were collected and made into smears.Conception was recorded after finding sperm in the smears,noting gestational day 0 (GD0).The pregnant mice were separated into three groups:control group,low level of vitamin D supplementation group and high level of vitamin D supplementation group.Different dosages of vitamin D supplementation were given by intramuscular injection from GD 15,with normal saline 0.25 ml/kg,vitamin D 20 μg/kg and vitamin D 100 μg/kg separately for each group.The injection continued until delivery.After 21 days breast-feeding,the thymus of filial generation mice were isolated.The expression of VDRmRNA was detected by Real-time PCR and the expression of VDR protein was detected by Western blot. Results No statistic difference of VDR gene expression was observed among different groups by Real-time PCR(P＞0.05).Western blot showed that the expression of VDR protein in low level of vitamin D supplementation group and high level of vitamin D supplementation group was higher than that of the control group (P＜0.05). Conclusion The expression of VDR in filial generation mice increases if mother mice are given vitamin D supplementation during pregnancy and is positively related to the dosage of vitamin D supplementation.

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vitamin D / vitamin D receptor / thymus

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WANG Zhi-hui,ZHAO Xia-xia,REN LI-hong.. Effect of vitamin D receptor expression in the thymus of filial generation mice after giving pregnant vitamin D.[J]. Chinese Journal of Child Health Care. 2016, 24(7): 712-714 https://doi.org/10.11852/zgetbjzz2016-24-07-11

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