Objective To investigate the hearing change and the characteristic of hearing thresholds of the infants failed in the hearing screening. Methods Totally 109 infants failed in the hearing screening were referred to People's Hospital of Xinjiang Uygur Autonomous Region.The Objective hearing of the infants were evaluated by the auditory brainstem response(ABR) and tympanometry. Results Finally 17( 15.6%) infants were normal hearing subjects and 92( 84.4%) showed hearing loss,including 52(47.7%)with milder hearing loss,17(15.6%)with moderate hearing loss,8 infants (7.4%) with severe hearing loss and 15 infants (13.7%) with very severe hearing loss.23 cases( 21.1%) of conductive hearing loss and 69 cases( 63.3%) of sensorineural hearing loss. Conclusions Development of auditory system and middle ear function in infants are the important factors in hearing screening.ABR and tympanometry for hearing evaluation and diagnose could improve the detection rate and provide useful information for early identification and intervention in this population.However,poor follow-up compliance and high loss of follow-up rate of Uygur are still big problems demanding urgent resolution.