Objective To explore the epidemiological characteristics of group A rotavirus (RV) and norovirus (NoV) infection among children under the age of five with diarrhea in Wuwei city. Methods The stool specimens were collected from children's outpatients with diarrhea in two sentinel hospitals from 2012 to 2014.Group A RV was detected by enzyme-linked immunosorbent assay (ELISA),and the positive specimens were used to make G/P genotyping with reverse transcription polymerase chain reaction (RT-PCR).The NoV was also detected by RT-PCR. Results A total of 182 stool samples were collected,the RV antigen-positive was 53 strains,and the positive rate was 29.12%.The difference between male and female was statistically significant (χ²=182.000,P<0.001).The cases were mainly concentrated in children aged 0~23 months.There was statistically significant difference in the positive rate between high-risk age group and the low-risk age group (χ²=219.576,P<0.001).The peak appeared in May and November,and November had the highest positive rate.There was statistically significant difference in the positive rate in high-risk month and the low-risk month (χ²=279.000,P<0.001).For the G/P genotyping,the style of G9,G3,P[8] were the main epidemic strains.While the analysis of G,P type combination mode showed the main epidemic strains were G9+P[8],followed by the G3+P[8].Additionally,the NoV antigen-positive rate was 15.93%.All cases were children under the age of two.In one year,the incidence of NoV also had two peaks,which were in April and December.There was also statistically significant difference in positive rates among the high-risk month and the low-risk month (χ²=159.641,P<0.001).In the taping of the two NoV positive specimens,one was GI accounted for 55.17%.One was GII,accounted for 44.83%.And the mixed infection positive rate of group A RV and NoV was 6.04%. Conclusion The group A RV and NoV are the main pathogens for children under the age of five infected diarrhea in Wuwei city,the related prevention work should be carried out according to the epidemiological characteristics.