Objective To investigate the changes of Notch-related protein hairy and enhancer of split 1 (Hes1) and astroglia marker glial fibrillary acidic protein (GFAP) and discuss effect of Notch signals in the rat model of autism. Methods Fifteen female Wistar rats (250~260 g) out of 20 were selected randomly to receive a single intraperitoneal injection of sodium valproate (VPA) at embryonic day 12.5(E12.5),and their offspring were defined as the autism model group.The rest 5 female Wistar rats received the intraperitoneal injection of saline,and their offspring were defined as the control group.The successfully animal model was confirmed by the three-chamber sociability test and self-grooming test.Western blotting was used to detect the expression of Hes1 and GFAP in prefrontal cortex in the two group on postnatal day 42(P42). Results 1)The animal model of autism was successfully established.Compared with the control group,the sociability and preference for social novelty were significantly decreased,and the cumulative self-grooming time in the autism model group significantly increased (P<0.05).2)Compared with the control group,Hes1 and GFAP expression significantly increased in prefrontal cortex of the autism model group on P42 (P<0.05). Conclusion In prefrontal cortex of the rat model of autism,activation of Notch signaling increased,moreover,the number of astrocyte also increased,which suggest the pathogenesis of autism may involve Notch signal in the prefrontal cortex.
Key words
autism /
Notch /
hairy and enhancer of split 1 /
glial fibrillary acidic protein
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