Effects on long-lasting social-related behavior in early postnatal fluoxetine treated on zebrafish.

WEN Zhu; LI Qiang; DU Ya-song; GUO Ning.

doi:10.11852/zgetbjzz2015-23-12-11

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Chinese Journal of Child Health Care ›› 2015, Vol. 23 ›› Issue (12) : 1267-1271. DOI: 10.11852/zgetbjzz2015-23-12-11

Effects on long-lasting social-related behavior in early postnatal fluoxetine treated on zebrafish.

WEN Zhu^1,2,LI Qiang³,DU Ya-song^1,2,GUO Ning⁴.

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Abstract

Objectives To expolore the effects toserotonin level and long-term social-related behaviour via a transient early postnatal fluoxetine exposure on zebarafish larves.Methods The level of neurotransmitters were exmained and their respective metabolites were quantified by HPLC from whole brain of larves (3~6 days post fertization)exposure to fluoxetine as well as tested the long-term social-related behaviour on zebrafish (30 days post fertization)quantified by social preference test.Results The level of 5-HIAA increased by enhancing dosage of fluoxetine,whlie the level of 5-HT,DA,DOPAC increased in low does and deceased in high dose.In social preference test,compared to the control group (1 255.50±117.95)cm,the 4 μM group (718.20±135.48)cm and 8 μM group (604.66±81.16) cm swam more distance in the area adjacent to the fish school(P<0.01);compared to the control group (1 005.61±54.86)s,the 4 μM group(754.21±85.63)s and 8 μM group(678.58±55.31)s had more time to swim in the area adjacent to the fish school(P<0.05). Conclutions The early-life exposed zebrafish shows the impaired long-term social-related behavior which may lead to sustained effect onneural development possibly via the changed level and function of the serotonin system.

Key words

zebrafish / fluoxetine / high precision liquid chromatography / social-related behaviour / autism spectrum disorder

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WEN Zhu,LI Qiang,DU Ya-song,GUO Ning.. Effects on long-lasting social-related behavior in early postnatal fluoxetine treated on zebrafish.[J]. Chinese Journal of Child Health Care. 2015, 23(12): 1267-1271 https://doi.org/10.11852/zgetbjzz2015-23-12-11

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