Objective To identify the status of serum vitamin A,D,E levels of infants and young children and to provide reference for clinical diagnosis and treatment. Methods Totally 67 healthy infants and young children were recruited for the study from January to March in 2013.Serum vitamin A,25-hydroxy vitamin D[25(OH)D],vitamin E were determined.Basic situation,feeding methods,physical and other aspects of the information were collected. Results Altogether 67 children,included 40 boys and 27 girls.The mean serum vitamin A was (0.23±0.07) mg/L,the rate of lack was 34.33%.Stratified analysis showed serum levels of different age had differences (F=5.866,P=0.001).Serum vitamin A deficiency rate gradually decreased with the month age increase.The 0~4 months of age group was the lowest with (0.17±0.04) mg/L,the lack rate was high as 68.75%.There were also differences in serum vitamin levels of different feeding methods (F=2.838,P=0.045),serum levels of exclusively breastfed infants was minimum with (0.18±0.03) mg/L,the lack rate was 60.00%.Serum 25(OH)D level was (102.26±90.54) nmol/L,the lack rate was 13.33%.There were differences between the different feeding methods (F=2.951,P=0.038).Serum levels of exclusively breastfed infants was low with (96.93±20.42) nmol/L,the higher rate of lack was 33.34%.Serum vitamin E level was (10.09±2.76) mg/L,rate of insufficient was only 13.4%,and there were no children.Vitamin E was better overall nutritional status. Conclusions Children's nutrition of vitamin A and 25(OH)D situations were not optimistic.The attention of the government should be improved to timely applying intervention to prevent vitamin deficiency for high-risk groups.