Objective To explore the relationship between rs9939609,rs8050236 sites of FTO gene with simple obesity. Methods A total of 208 children (aged from 3 to 18) in Liuzhou were enrolled.Body height,weight and waistline were measured.Serum fasting plasm glucose,cholesterol,triglyceride,high-density lipoprotein and low-density lipoprotein were measured.FTO gene rs9939609 (A/T) and rs8050136 (C/A) sites single nucleotide polymorphisms were detected by SNaPshot assay. Results The obesity group showed the components of the metabolic parameters (FBG,TC,TG,HDL-C and VDL-C) were significant differences compared to control group.The TT,AT and AA genotype frequencies of FTO gene rs9939609(A/T) in simple obesity group and normal control group were 79.85%,17.16%,2.99% and 71.52%,25.17%,3.31%,respectively,the T,A allelic frequencies in simple obesity group and normal control group were 88.43%,11.57% and 84.10%,15.89%,respectively.And OR was 1.45,95%CI was 0.89~2.35.The CC,CA and AA of rs8050136(C/A) were 77.61%,19.40%,2.97% and 70.76%,25.83%,3.31%,respectively.The C,A allelic frequencies in simple obesity group and normal control group were 87.31%,12.69% and 83.77%,16.23%,respectively.And OR was 1.333,95%CI was 0.831~2.137.The genotype and allelic frequencies of rs9939609 (T/A) and rs8050136 (C/A) in simple obesity children were not significantly different from those in controls (P>0.05),the two sites single nucleotide polymorphisms were found in Hardy Weinberg equilibrium.There was no significant association between the two sites (rs9939106 and rs8050136) and obestity-related traits including FBG,TC,TG,HDL-C and VDL-C. Conclusions FTO gene rs9939609 and rs8050136 polymorphisms are not associated with simple obesity in Chinese Zhuang children in Liuzhou of Guangxi province,and there was no significant association among the polymorphisms of two sites and metabolic parameters.
Key words
genetic polymorphism /
obesity /
children of Zhuang nationality
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