Objective To study the effect of ethyl pyruvate(EP) in the expression of 12-lipoxygenase(12-LOX) and apoptosis changes on rat's offspring's damaged brain caused by intrauterine infection. Methods Pregnant wistar rats of lipopolysaccharide(LPS) group were consecutively injected with lipopolysaccharide [380 μg/(kg·day)] intraperitoneally.Pregnant Wistar rats of EP group were consecutively injected with LPS intraperitoneally,and EP was administrated intraperitoneally at a dose of 40 mg/kg immediately.12 pregnant rats were injected with normal saline intraperitoneally as control group.After the delivery of pups,thirty-six pups were selected in every group randomly.placenta from rats of LPS group was taken for hematoxylline-eosin (HE) staining to confirm its intrauterine infection.12-LOX expression in brain tissue was studied through immunohistochemical and western-blot analysis.TUNEL was used to observe the pathological changes of the brain tissue. Results Obvious pathological changes were observed in the placenta in the infection groups.The expression of apoptosis was lower in EP group and NS group than in the LPS group (P<0.05),and lower in NS group than that in the EP group (P<0.05).The expression of 12-lipoxygenase was lower in EP group and NS group than that in the LPS group (P<0.05),and lower in NS group than that in the EP group (P<0.05). Conclusion EP confers potent protection against LPS-induced brain injury via significantly reduced the expression of 12-LOX and anti-apoptotic properties.
Key words
ethyl pyruvate /
intrauterine infection /
brain injury /
12-lipoxygenase /
apoptosis
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