Objective To study the cell apoptosis and expression of Omi/HtrA2 in brain of neonatal rats after asphyxia and to evaluate the effects of Salvia miltorrhiza bunge( SMB ) on it. Methods A total of 75 SD rats aged 7 days were randomly divided into the following groupscontrol group, asphyxia group and SMB treatment group, then the brain tissues were taken from the rats in the three groups at 6, 12, 24, 48 hours and 72 hours after the asphyxia.The apoptotic cells were detected by the TUNEL staining method, The Omi/HtrA2 protein expression was detected with the immunhistochemistry method. Results The number of apoptotic cells and the expression of Omi/HtrA2 were higher in asphyxia groups than those in control group (P<0.05).In asphyxia groups, the expression of Omi/HtrA2 reached peak at 12 h after asphyxia(10.98±1.34), and the number of apoptotic cells reached peak at 24 h after asphyxia (12.1±2.66).But compared with asphyxia group, the expression of Omi/HtrA2 and the number of apoptotic cells were significantly decreased in SMB treatment group, and the differences were significant(P＜0.05), but all parameters were not restored to the levels of control group(P＜0.05). Conclusion Asphyxia can induce the apoptosis of neuronal, Omi/HtrA2 may be involved in this process.SMB can reduce the number of apoptotic cells by inhibiting the expression of Omi/HtrA2 in brain, and thus it can play a protective role in asphyxia brain damage of neonatal rats.
Key words
asphyxia /
neonatal rat /
neuron /
apoptosis /
Omi/HtrA2 /
Salvia miltiorrhiza Bunge
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