Objective To investigate the effects of erythropoietin on vascular endothelial growth factor(VEGF) and nuclear factor kappa B (NF-κB )expression in neonatal rats with hypoxic-ischemic brain damage. Methods The 7 days old rats were randomly divided into three groups (n=24 in each group):sham-operated group,HIBD group and EPO-treated group.In sham-operated group,the median incision of neck was performed,and left common carotid artery was isolated,but not hypoxia-ischemia.In HIBD group,left common carotid artery was isolated and ligated,then the models were prepared under hypoxia for 2 h.In EPO-treated group,the model rats were instantly given single intraperitoneal injection of rhEPO (5 000 U /kg).The expression of VEGF and NF-κB were detected by immunohistochemistry at 6,12,24,72 hours after the operation. Results 1) Expression of VEGF was higher in HIBD group than in sham-operated group.Expression of VEGF was significantly higher in EPO-treated group than in sham-operated group and HIBD group at the same time (P<0.01).2) Expression of NF-κB was higher in HIBD group than in sham-operated group.Expression of NF-κB was higher in HIBD group than in EPO-treated group at the same time (P<0.01). Conclusion Erythropoietin can up-regulate expression of VEGF and reduce expression of NF-κB in neonatal rats after hypoxic-ischemic brain damage,which may be one of the mechanisms for protecting HIBD.
Key words
erythropoietin /
vascular endothelial growth factor /
nuclear factor kappa B /
hypoxia ischemia /
neonatal rats
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