Objective To explore the therapeutic effect of transplantation of bone marrow stromal cells (BMSCs) on learning and memory ability of hypoxic ischemic rats and its related mechanism. Methods SD rats' BMSCs were cultured from femur in vitro,and labeled with BrdU,then implanted into 7 days old hypoxic-ischemic rats,the water maze experiment was tested after 8 weeks,then rats' brains were fixed,dehydrated,embedded,sliced,then did immunohistochemical staining and detected BrdU. Results Morris water maze showed T1 and T2 of the model group were shorter than those of the sham operation group (P＜0.05).T1 and T2 of cell group were longer than those of the model group (P<0.05).The integral optical density(IOD) value of immunoreative positive cells of NSE in model group was significantly lower than that of sham operation group (P＜0.05),IOD of cell group was higher than that of the model group (P＜0.05).But BrdU positive substance in cell group was bare. Conclusions Implanted BMSCs through lateral ventricle can improve the learning and memory ability of rats.The mechanism may be that BMSCs and related material injected into the lateral ventricle can promote neuron to regenerate.
Key words
bone marrow stromal cells /
transplantation /
hypoxic /
ischemia /
rat /
memory
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