Objectiv To compare the distribution and differentiation of human umbilical cord mesenchymal stem cells(hUCMSCs) after the transplantation via the jugular vein and via the intraperitioneal injection,and investigate the effect of hUCMSCs transplantation on the recovery of neurological functions after hypoxic-ischemic brain damage (HIBD). Methods The models of 7-day-old neonate rats with HIBD brain were established,and randomly assigned to:HIBD group (n=10),the intravenous injection group (n=15),the intraperitioneal injection group (n=15) and control group (n=10),the HIBD group and control group were not transplanted.The same amount hUCMSCs(1×106,0.2 mL)were transplanted into the intravenous injection group and the intraperitioneal injection group after 72 hours.Finally,brain tissues of rats were removed after 4% paraformaldehyde was perfused in cardiac to fix brain and successive sections were made using a cryomicrotome.Immunofluorescence staining was performed to examine the distribution of Dil-labeled hUCMSCs and expression of DCX. Results The transplantation descendants of hUCMSCs could migrate to the brain tissue,and could differentiate into neuronal precursor cells,and the intravenous injection group showed more Dil-labeled hUCMSCs in brain tissue. Conclusions Transplanted hUCMSCs can survive and differentiate in the HIBD rats' brain.The HUCMSCs can effectively accelerate the recovery of neurological functions after HIBD.And the experiment proved intravenous injection is better than intraperitioneal injection in repairing rats' brain function.
Key words
human umbilical cord mesenchymal stem cells /
hypoxic-ischemic brain damage /
transplantation /
intravenous injection /
intraperitioneal injection
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References
[1] Seshareddy K,Troyer D,Weiss ML.Method to isolate messenchymal-like cells from Wharton's Jelly of umbilical cord[J].Methods Cell Biol,2008,86:101-119.
[2] Moretti P,Hatlapatka T,Marten D,et al.Mesenchymal stromal cells derived from human umbilical cord tissues:primitive cells with potential for clinical and tissue engineering applications[J].Adv Biochem Eng Biotechnol,2010,123:29-54.
[3] Allison M.Genzyme backs osiris,despite prochymal flop[J].Nat Bioteehnol,2009,27(11):966-977.
[4] Bentlage C,Nikkhan G,Cunningham MG,et al.Reformation of the nigrostriatal pathway by fetal dopaminergic micrografts into the substantia nigra is critically dependent on the age of the host[J].Exp Neurol,1999,159(1):177-190.
[5] Jansen EM,Solberg L,Underhill S,et al.Transplantation of fetal neocortex ameliorates sensorimotor and locomotor deficits following neonatal ischemic-hypoxic brain injury in rats[J].Exp Neurol,1997,147(2):487-497.
[6] Park KI.Transplation of neural stem cells:cellular and gene therapy for hypoxic-ischemic brain injury[J].Yonsei Med J,2000,41(6):825-835.
[7] 官晓清,余加林.骨髓间充质干细胞透过新生大鼠血脑屏障的实验研究[J].中华儿科杂志,2004,42(12):920-923.
[8] Wang L,Li Y,Chen X,et al.MCP-Ⅰ,MIP-Ⅰ,IL-8 and ischemic cerebral tissue enhance human bone marrow stromal cell migration in interface culture[J].Hematology,2002,7(2):113-117.
[9] Dormady SP,Bashayan O,Dougherty R,et al.Immortablized multipotential mesenchymal cells and the hematopoietic microenvironment[J].J Hematother Stem Cell Res,2001,10(1):125-140.
[10] Kim SS,Yoo SW,Park TS,et al.Neural induction with neurogenin 1 increases the therapeutic effects of mesenchymal stem cells in the ischemic brain[J].Stem Cells,2008,26(9):2217-2228.
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