Objective To observe the effect of human umbilical cord mesenchymal stem cells (hUCMSCs) combined with ganglioside (GM1) on the treatment of hypoxic-ischemic brain damage (HIBD) in neonate rats. Methods First, the models of 7-day-old neonate rats with HIBD brain were established.Then, after 72 hours, the same amount hUCMSCs were transplanted into the hUCMSCs transplantation group and the hUCMSCs+GM1 group, at the same time, GM1 was injected into the hUCMSCs+GM1 group through abdominal cavity.Spatial learning was assessed using a Morris water maze test from 31 to 35 days after the transplantation.Finally, after the behavioral tests, brain tissues of rats were removed after 4% paraformaldehyde was perfused in cardiac to fix brain and successive sections were made using a cryomicrotome.Immunofluorescence staining was performed to examine the distribution of DiI-labeled hUCMSCs and expression of DCX. Results The hUCMSCs+GM1 group showed more significant neurological functions improvement than the hUCMSCs transplantation group as revealed by Morris water maze test.The transplantation descendants of hUCMSCs could migrate to the brain tissue, and could differentiate into neuronal precursor cells, and the hUCMSCs+GM1 group showed more DiI-labeled hUCMSCs in brain tissue. Conclusion Transplanted hUCMSCs could improve the recovery of neurological functions after HIBD and promote rats' behavioral function improvement.And GM1 exerts a synergistic effect in this treatment.
Key words
human umbilical cord mesenchymal stem cells /
hypoxic-ischemic brain damage /
transplantation /
ganglioside
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