Objective To explore whether high fat diet-induced obesity and leptin resistance are attenuated by silencing suppressor of cytokine signaling 3(SOCS3) by means of RNA interference(RNAi) in young rats. Methods A total of 32 five-week-old Sprague-Dawley (SD) rats were divided into 4 groups randomly.The lentiviral vector(LV)-SOCS3-shRNA(RNAi) or LV-control-shRNA(Con) were stereotaxically injected bilaterally into the lateral ventricle of rats which then fed with hight-fat diet(HFD) or chow diet (CD) for 28 days, designated as RNAi+CD group, Con+CD group, RNAi+HFD group and Con+HFD group.Food intake and body weight were measured every 2 days and 7 days respectively.Up to 28 days after treatment, blood was harvested from the four group rats.Then levels of leptin, plasma glucose (PG), total cholesterol (TC) and triglycerides (TG) were detected by radioimmunity assay or automatic biochemistry analyzer respectively. Results Cumulative food intake and body weight of rats treated with RNAi+HFD exhibited decrease compared with those treated with Con+HFD after injection of 14 days and 21 days respectively (P＜0.05), but showed increase compared with those treated with Con+CD after injection of 22 days and 28 days respectively (P＜0.05).Level of leptin in rats treated with RNAi+HFD (0.86±0.12) ng/mL was significantly lower than those treated with Con+HFD (0.97±0.13) ng/mL and higher than those treated with Con+CD(0.75±0.10)ng/mL (P＜0.05). Conclusions Knockdown of SOCS3 by RNAi partly relieves high fat diet-induced obesity and leptin resistance in young rats.
Key words
obesity /
young rats /
RNA interference /
suppressor of cytokine signaling 3
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