Tracing study on the neurobehavioral effects of neonatal rats by the intrauterine human cytomegalovirus infection.

PAN Na-na; WANG Li-li; WU De; TANG Jiu-lai.

doi:10.11852/zgetbjzz2014-22-06-13

PDF(740 KB)

Chinese Journal of Child Health Care ›› 2014, Vol. 22 ›› Issue (6) : 600-604. DOI: 10.11852/zgetbjzz2014-22-06-13

Tracing study on the neurobehavioral effects of neonatal rats by the intrauterine human cytomegalovirus infection.

PAN Na-na¹, WANG Li-li¹, WU De², TANG Jiu-lai².

Author information +

History +

Abstract

Objective To detect the dynamic influence of nervous system development in neonatal rats by the intrauterine human cytomegalovirus (HCMV) infection. Methods The SD rat model of intrauterine HCMV infection was established, then the rats selected from experimental group and the control group randomly were done neuroethology test, such as Morris water maze test, hanging test, slope test, the open field test etc at the age of 1, 3 and 6 months respectively.Before and after the experiment, the light microscope and electron microscopy were used to observe their dynamic pathological change of brain tissue separately. Results Compared with normal control group, the Morris water maze escape latency, swimming distance of the experimental group at the age of 1, 3, 6 months were significantly higher than those of the control group, at the same time cross the platform number was significantly lower than control group, and with the increase of age, the cross platform ability was drop obviously (F=45.09, P<0.01);Motor functionrats' hanging test scores were (1.49±0.53), (1.23±0.46), (0.93±0.35) in infected group at the age of 1, 3 and 6 months, while those in the control group were (2.29±0.49), (2.14±0.69), (1.98±0.25), there were significant differences between two groups(samples nonparametric test, P all<0.001), suggested sport function of infected neonatal rat was decreased significantly.Open filed resultoffspring's across the grid number were (44.0±7.32), (37.40±9.56), (26.62±8.87) in infected group in 1, 3 and 6 months of age, while those in the control group were (57.07±9.65), (55.27±9.36), (54.27±8.14), there were significant differences between two groups (P all <0.05).Pathological results showed that along with the age growth, the hippocampus structure and nerve myelin of the experimental group offspring were aggravate gradually. Conclusion Intrauterine HCMV infection may result in the abnormal neural behavior of neonatal rat, and the impairment of learning and memory, motor function and emotional behavior development is aggravate gradually.

Key words

human cytomegalovirus / intrauterine infection / neonatal rats / neurobehavior

Cite this article

EndNote

Ris (Procite)

Bibtex

Download Citations

PAN Na-na, WANG Li-li, WU De, TANG Jiu-lai.. Tracing study on the neurobehavioral effects of neonatal rats by the intrauterine human cytomegalovirus infection.[J]. Chinese Journal of Child Health Care. 2014, 22(6): 600-604 https://doi.org/10.11852/zgetbjzz2014-22-06-13

References

[1] Buonsenso D, Serranti D, Gargiullo L, et al.Congenital cytomegalovirus infection:current strategies and future perspectives[J].Eur Rev Med Pharmacol Sci, 2012, 16(7):919-935.
[2] Swanson EC, Schleiss MR.Congenital cytomegalovirus infection:new prospects for prevention and therapy[J].Pediatr Clin North Am, 2013, 60(2):335-349.
[3] 李天宪, 赵林, 冯峰.人巨细胞病毒小鼠模型的建立[J].微生物学报, 1996, 36(4):292-294.
[4] Tang JL, Wang ML, Qiu JJ, et al.Building a mouse model hallmarking the congenital human cytomegalovirus infection in central nervous system[J].Arch Virol, 2002, 147(6):1189-1195.
[5] Lombardi G, Garofoli F, Mauro Stronati et al.Congenital cytomegalovirus infection:treatment, sequelae and follow-up[J].J Matern Fetal Neonatal Medi, 2010, 23(S3):45-48.
[6] 倪坤, 唐久来, 吴德, 等.孕早期人巨细胞病毒感染对仔鼠海马N-甲基-D-天冬氨酸受体亚基表达的影响[J].实用儿科临床杂志, 2012, 27(2):913-915.
[7] Jiang PF, Zhu T, Gao JD, et al.The effect of maternal infection on cognitive development and hippocampus neuronal apoptosis, proliferation and differentiation in the neonatal rats[J].Neuroscience, 2013, 246:422-434.
[8] Yoshihiro T, Hideyas, Kawasaki, et al.Reactivation of latent cytomegalovirus infection in mouse brain cell detected after Transfer to brain slice culture[J].Journal of Virology, 2002, 76(14):7247-7254.
[9] Townsend CL, Marianne FM, Ahlfors K, et al.Long-term outcomes of congenital cytomegalovirus infection in Sweden and the United Kingdom[J].Clin Infect Dis, 2013, 56(9):1232-1239.
[10] Bosnjak VM, Dakovi

I, Duranovi

V, et al.Malformations of Cortical Development in children with congenital cytomegalovirus infection -a study of nine children with proven congenital cytomegalovirus infection[J].Coll Antropol, 2011, 35(S):229-234.
[11] 孙竹风, 陈英.先天性巨细胞病毒感染导致脑损伤的临床分析[J].中国优生与遗传杂志, 2008, 16(5):123-125.
[12] Kawatani M, Nakai A, Okuno T, et al.Detection of cytomegalovirus in preserved umbilical cord from a boy with autistic disorder[J].Pediatr Int, 2010, 52(2):304-307.
[13] Yamashita Y, Fujimoto C, Nakajima E, et al.Possible association between congenital cytomegalovirus infection and autistic disorder[J].J Autism Dev Disord, 2003, 33(4):455-459.