Expression of cytoglobin in hypoxic neurons in vitro

MA Hai-ran; SHI Xue-chuan

doi:10.11852/zgetbjzz2014-22-05-14

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Chinese Journal of Child Health Care ›› 2014, Vol. 22 ›› Issue (5) : 492-496. DOI: 10.11852/zgetbjzz2014-22-05-14

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Expression of cytoglobin in hypoxic neurons in vitro

MA Hai-ran,SHI Xue-chuan

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Abstract

Objective To examine the expression of cytoglobin(CYGB) of neurons in vitro when suffered hypoxia,which provides an experimental basis for exploring the physiological and pathological mechanism and seeking new treatments of hypoxic-ischemic encephalopathy. Methods The primary cortical neurons from embryonic rat were cultured in a serum-free culture system of B27-supplemented neurobasal medium.The neurons were identified by polyclonal antibody against neuron specific enolase(NSE) on the 6th day.They were divided into 4 groups randomly for assessing cell viability by CCK8 and observing the expression of CYGB by Real-time PCR and Westernblot at 0、8、16、24 h after hypoxic model(oxygen concentration between 0.5% and 0.9%) was induced. Results The purity of neurons was greater than 99%;and cell viability were slightly reduced after hypoxia.Expression of RNA and protein of CYGB was increased in a time-dependent manner.And there was statistical differenc in each group (P<0.05). Conclusions CYGB can up-regulate in neurons in vitro after hypoxia,indicating CYGB may play certain important roles in neurons under hypoxia.

Key words

cytoglobin / neurons / hypoxic-ischemic encephalopathy

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MA Hai-ran,SHI Xue-chuan. Expression of cytoglobin in hypoxic neurons in vitro[J]. Chinese Journal of Child Health Care. 2014, 22(5): 492-496 https://doi.org/10.11852/zgetbjzz2014-22-05-14

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