目的 探讨慢性间断性低氧(chronic intermittent hypoxia,CIH)对幼鼠肾脏超微结构和功能的影响。 方法 雄性21日龄SD幼鼠22只,体质量(50±5)g,随机均分为对照组和慢性间断性低氧组(CIH组)。CIH组每日间断缺氧8 h,连续缺氧6周。利用透射电镜观察肾脏超微结构变化;利用比色法测定血清丙二醛(malondialdehyde,MDA)、肾组织丙二醛水平,测定尿N-乙酰-β-D氨基葡萄糖苷酶(NAG)活性和肌酐(Cr)含量。 结果 透射电镜观察发现对照组肾脏超微结构基本正常,CIH组肾小球内皮细胞水肿,足细胞突起融合,肾小管上皮细胞水肿明显。与对照组比较,CIH组血清MDA、肾组织MDA水平明显升高(P<0.01)。与对照组比较,CIH组尿NAG活性明显升高(P<0.01),尿Cr含量明显降低(P<0.05)。 结论 CIH可导致幼鼠肾脏超微结构改变和肾功能损害,这可能与缺氧后产生过多的氧自由基有关。
Abstract
Objective To investigate the effect of chronic intermittent hypoxia (CIH) on renal ultrastructure and function in immature rats. Methods A total of 22 postnatal day 21 (P21) Sprague-Dawley (SD) male rats were randomly averagely divided into two groups:control group and CIH group.CIH-handled rats were exposed to intermittent hypoxia in designed cabin 8 hours every day,and the duration of hypoxia was 6 weeks.The renal ultrastructure was observed by transmission electron microscopy.The value of serum malondialdehyde (MDA) and renal malondialdehyde,activity of urinary enzyme N-β-D-glucosaminidase as well as content of the urinary creatinine were detected by colorimetry. Results The renal ultrastructure of control group were normal.The glomerular endothelial cells showed edema and the foot process fusion were demonstrated in CIH group.Renal tubular epithelial cells appeared obvious edema in CIH group.Compared with the control group,the value of serum MDA and renal MDA were increased obviously (P<0.01).There were significantly increased in activity of Urinary N-acetyl-D-glucosaminidase (NAG) of CIH group (P<0.01),but the content of the urinary creatinine were less than those in control group (P<0.05). Conclusion The study demonstrated CIH exposures result in changes of renal ultrastructure and impairment of renal function,which may be associated with the produce of excessive oxygen radicals after hypoxia.
关键词
低氧 /
肾脏 /
超微结构 /
功能 /
幼鼠
Key words
hypoxia /
kidney /
ultrastructure /
function /
immature rats
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