间断性低氧对幼鼠认知功能及海马突触超微结构的影响
- 陈燕1,赵春玲1,余广1,程基焱2
作者信息
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Effects of intermittent hypoxia on cognition and the ultrastructure of hippocampal synapses in immature rats.
- CHEN Yan1,ZHAO Chun-ling1,YU Guang1,CHENG Ji-yan2.
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文章历史
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摘要
目的 观察慢性间断性低氧(chronic intermittent hypoxia,CIH)对幼鼠认知功能及海马突触超微结构的进行性影响。 方法 雄性21日龄SD幼鼠66只,体重(50±5)g,随机分为正常对照组(unhandled control group,UC组)和慢性间断性低氧组(CIH组),每组33只,每组又分为2,4,6周3个时间点,每个时间点11只幼鼠。CIH组每日间断缺氧8 h,通过Morris水迷宫测试幼鼠学习和记忆能力,利用透射电镜观察海马CA1区突触超微结构的变化。 结果 1)Morris水迷宫定位航行试验:CIH组幼鼠逃避潜伏期明显长于UC组,两组之间差异具有统计学意义(P<0.05)。2)Morris水迷宫空间搜索试验:与UC组比较,CIH组跨越目标象限时间占整个游泳时间的百分率明显减少,差异具有统计学意义(P<0.05)。与UC组比较,CIH组穿越平台次数明显减少,差异具有统计学意义(P<0.05)。3)电镜下可见CIH组突触小泡数量减少,结构不清,线粒体出现空泡样改变。UC组无明显病变。 结论 CIH致幼鼠认知功能进行性下降与海马CA1区突触超微结构的改变密切相关。
Abstract
Objective To study the progressive effects of chronic intermittent hypoxia(CIH) on cognition and the ultrastructure of hippocampal synapses in immature rats. Methods A total of Sixty-six healthy,male,21-day-old Sprague-Dawley immature rats,weighing (50±5) g,was randomly averagely divided into two groups:unhandled control(UC)group and CIH group.Every group was divided into three time points:2,4 and 6 weeks(n=11).CIH-handled immature rats were exposed to intermittent hypoxia in designed cabin 8 hours every day.The cognitive function was assessed by the Morris Water Maze(MWM).The ultrastructure of hippocampal synapses were observed by electron microscope. Results 1)MWM of place navigation test:the escape latency in CIH immature rats was significantly longer compared with that in UC rats(P< 0.05);2)MWM of spatial probe test:the percentage of time spent on crossing the target quadrant to the total swimming time in CIH group was significantly decreased compared with that in UC group(P<0.05).The number of times of crossing the platform in CIH group was significantly reduced compared with that in UC group(P<0.05);3)Changes in the ultrastructure of hippocampal synapses were obvious in CIH group as compared with the UC group. Conclusion Chronic intermittent hypoxia induced slowly progressive cognition impairments in immature rats,which maybe associated with the changes in the ultrastructure of hippocampal synapses exposed to CIH.
关键词
Key words
hypoxia / cognition / hippocampus / ultrastructure / synapses / immature rats
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参考文献
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[2] Liner LH,Marcus CI.Ventilatory management of sleep-disordered breathing in children[J].Curr Opin Pediatr,2006,18(3):272-276.
[3] Sans-Capdevila O,Gozal D.Neurobiological consequences of sleep apnea syndrome in children[J].Rev Neurol,2008,47(12):659-664.
[4] Torelli F,Moscufo N,Garreffa G,et al.Cognitive profile and brain morphological changes in obstructive sleep apnea[J].Neuroimage,2011,54(2):787-793.
[5] Lurie A.Obstructive sleep apnea in adults:epidemiology,clinical presentation,and treatment options[J].Adv Cardiol,2011,46:1-42.
[6] Rajagopalan N.Obstructive sleep apnea:not just a sleep disorder[J].J Postgrad Med,2011,57(2):168-175.
[7] 杨宇,谭胜玉,张新民,等.间歇性缺氧对大鼠认知功能和海马CA1区超微结构的影响[J].中国神经免疫学和神经病学杂志,2007,3(14):157-163.
[8] Iturriaga R,Moya EA,Del Rio R.Cardiorespiratory alterations induced by intermittent hypoxia in a rat model of sleep apnea[J].Adv Exp Med Biol,2010,669(9):271-274.
[9] Ward CP,McCoy JG,McKenna JT.Spatial learning and memory deficits following exposure to 24 h of sleep fragmentation or intermittent hypoxia in a rat model of obstructive sleep apnea[J].Brain Res,2009,1294(6):128-137.
[10] Hoban TF.Sleep disorders in children[J].Ann N Y Acad Sci,2010,1184:1-14.
[11] 邱宏,金围琴,金如锋,等.水迷宫重复测量方差分析及其在SPSS中的实现[J].中西医结合学报,2007,5(1):101-105.
[12] Tatlipinar A,Duman D,Uslu C,et al.The effects of obstructive sleep apnea syndrome due to adenotonsillar hypertrophy on the cardiovascular system in children[J].Turk J Pediatr,2011,53(4):359-363.
[13] Sinha D,Guilleminault C.Sleep disordered breathing in children[J].Indian J Med Res,2010,131:311-320.
[14] Jiang W,Duong TM,de Lanerolle NC.The neuropathology of hyperthermic seizures in the rat[J].Epilepsia,1999,40(1):5-19.
[15] Hoffmann H,Hunt P,Spear NE.Ontogenetic differences in the association of gustatory and tactile cues with lithium chloride and footshock[J].Behav Neural Biol,1990,53(3):441-450.
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