目的 检测健康儿童心肌酶指标,建立本地区健康儿童各年龄组心肌酶谱正常参考范围。方法 对957例健康儿童采集周围静脉血采用速率法在美国雅培C-8000全自动生化分析上测定AST、CK、CK-MB、LDH、α-HBDH血清水平。结果 心肌酶正常参考值随年龄增长而逐渐下降,血清AST、CK、CK-MB、LDH、α-HBDH水平差异有统计学意义(P＜0.05或＜0.01),Ⅰ组:24~168h(早期新生儿组)明显高于Ⅱ组:~3岁(婴幼儿组)、Ⅲ组:~6岁(学龄前组)、Ⅳ组:~12岁(学龄期组);儿童各年龄组与成人对照组差异显著(P＜0.05或＜0.01),均显著高于成人对照组正常参考范围。儿童各年龄组正常参考范围:Ⅰ组 AST 23~113 U/L;CK 194~1104 U/L;CK-MB 25~115 U/L;LDH 140~956 U/L;α-HBDH 92~754 U/L;Ⅱ组 AST 19~73 U/L;CK 37~237 U/L;CK-MB 7~55 U/L;LDH 176~414 U/L;α-HBDH 122~320 U/L);Ⅲ组LDH 165~343 U/L、α-HBDH 102~274 U/L;Ⅳ组 AST 13~49 U/L;CK 20~186 U/L;CK-MB 5~39 U/L;LDH 126~286 U/L;α-HBDH 86~220 U/L。 结论 建立适合本地区新生儿、婴幼儿、学龄前及学龄期心肌酶谱的正常参考范围,为临床的诊断和治疗提供可靠的指标。

Objective To establish the normal reference ranges of myocardial enzyme series of each age group for the healthy children in Songzi city by testing the healthy children's myocardial enzymes. Methods 957 healthy children's peripheral venous blood were collected serum AST,CK,CK-MB,LDH and α-HBDH levels were determined by kinetic rate method on Architect C-8000 Aerosef. Results The normal reference value of myocardial enzyme was reduced gradually with aging.The differences of serum AST,CK,CK-MB,LDH and α-HBDH levels were significant among the four groups(P＜0.05 or ＜0.01).The levels of myocardial enzymes of GroupⅠ(24~168 h,newborn group)were obviously higher than of Group Ⅱ(~3 years old,infants group),Group Ⅲ(~6 years old,preschool group)and Group Ⅳ(~12 years old,school-aged group).The myocardial enzyme values of children in all groups were significantly higher than the normal reference ranges of the adults control group(P＜0.05 or ＜0.01).The normal reference ranges of myocardial enzymes in all of the four age groups were as follows:GroupⅠ,AST 23~113 U/L;CK 194~1104 U/L;CK-MB 25~115 U/L;LDH 140~956 U/L and α-HBDH 92~754 U/L;GroupⅡ,AST 19~73 U/L;CK 37~237 U/L;CK-MB 7~55 U/L;LDH 176~414 U/L and α-HBDH 122~320 U/L;Group Ⅲ,LDH 165~343 U/L and α-HBDH 102~274 U/L;Group Ⅳ,AST 13~49 U/L;CK 20~186 U/L;CK-MB 5~39 U/L;LDH 126~286 U/L and α-HBDH 86~220 U/L. Conclusion The establishment of adaptive normal reference ranges of myocardial enzyme series for the local newborns,infants,preschool children and school-aged children would provide reliable indexes for clinical diagnosis and treatment.