目的 探讨γ-维生素E(γ-T)对哮喘的治疗作用及机制。方法 将40只雄性Balb/c小鼠(4周,18~20 g)随机分为正常组、哮喘组、地塞米松组及γ-T治疗组。给予小鼠腹腔注射及雾化吸入卵白蛋白建立哮喘气道炎症反应模型,分别给予地塞米松及γ-T,正常组仅给予生理盐水。28 d后处死小鼠,留取血清及肺泡灌洗液。ELISA法测定各组血清及肺泡灌洗液中嗜酸性粒细胞趋化因子(eotaxin)的含量。用one-way ANOVA检验组间差异,P＜0.05具有统计学意义。结果 哮喘组小鼠血清及肺泡灌洗液中eotaxin浓度均较正常组升高,地塞米松组及γ-T组均较哮喘组下降。在血清中地塞米松组下降最明显,γ-T组次之,γ-T组与哮喘组,γ-T组与地塞米松组相比差异无统计学意义(P>0.05);在BALF中,γ-T组的eotaxin下降最明显,与哮喘组相比,二者间差异明显(P<0.05);γ-T组与地塞米松组相比,二者差异无统计学意义(P>0.05)。 结论 γ-T有利于减少哮喘小鼠肺组织eotaxin,γ-T可能是有用的抗哮喘药物。
Abstract
Objective To explore the effect and mechanism of gamma-tocopherol(γ-T) in asthmatic mice. Methods A total of 40 male Balb/c mice(4weeks,18~20 g) were randomly divided into four groups:asthma group,dexamethasone group,γ-T group,and normal group.The mice of asthma group were received intraperitoneal injection of OVA (10 μg) on day 0,7,14 and exposed to 25 g/L OVA inhalation from day 21 for one week.Ovalbumin (OVA)-sensitized mice were challenged with dexamethasone and γ-T for one week,the normal group were only given saline.Blood plasma and BALF samples were collected after the 28 d mice were sacrificed.The concentration of eotaxin in serum and BALF were determined by the method of ELISA.One-way ANOVA was used test the difference between groups. Results The concentration of eotaxin in serum and BALF of asthmatic mice were higher than normal,and those were lower in dexamethasone and γ-T group.The concentration of eotaxin in serum of γ-T group were lower compared with asthma and dexamethasone group(P>0.05);The concentration of eotaxin in BALF of γ-T group were lower compared with dexamethasone and asthma group(P all<0.05). Conclusion γ-T prohibit the expression of eotaxin in asthmatic mice,and γ-T might be an effective therapy of allergic airway inflammation.
关键词
哮喘 /
维生素E /
γ-维生素E /
嗜酸性粒细胞趋化因子
Key words
asthma /
vitamin E /
gamma-tocopherol /
eotaxin
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基金
2011辽宁省教育厅课题(L2011128)
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